Piezoelectric biosensors for real-time monitoring of hybridization and detection of hepatitis C virus

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dc.contributor.authorSkladal, P.
dc.contributor.authorRiccardi, C. D.
dc.contributor.authorYamanaka, Hideko [UNESP]
dc.contributor.authorda Costa, P. I.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionMasaryk Univ
dc.date.accessioned2014-05-20T13:24:24Z
dc.date.available2014-05-20T13:24:24Z
dc.date.issued2004-05-01
dc.description.abstractThe piezoelectric quartz crystal resonators modified with oligonucleotide probes were used for detection of hepatitis C virus (HCV) in serum. The gold electrodes on either rough or smooth surface crystals were modified with a self-assembled monolayer of cystamine. After activation with glutaraldehyde, either avidin or streptavidin were immobilized and used for attachment of biotinylated DNA probes (four different sequences). Piezoelectric biosensors were used in a flow-through setup for direct monitoring of DNA resulting from the reverse transcriptase-linked polymerase chain reaction (RT-PCR) amplification of the original viral RNA. The samples of patients with hepatitis C were analyzed and the results were compared with the standard RT-PCR procedure (Amplicor test kit of Roche, microwell format with spectrophotometric evaluation). The piezoelectric hybridization assay was completed in 10 min and the same sensing surface was suitable for repeated use. (C) 2004 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Inst Chem, Dept Analyt Chem, BR-14801900 Araraquara, SP, Brazil
dc.description.affiliationMasaryk Univ, Fac Sci, Dept Biochem, CS-61137 Brno, Czech Republic
dc.description.affiliationUniv Estadual Paulista, Fac Pharmaceut Sci, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Chem, Dept Analyt Chem, BR-14801900 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Pharmaceut Sci, BR-14801902 Araraquara, SP, Brazil
dc.format.extent145-151
dc.identifierhttp://dx.doi.org/10.1016/j.jviromet.2004.01.005
dc.identifier.citationJournal of Virological Methods. Amsterdam: Elsevier B.V., v. 117, n. 2, p. 145-151, 2004.
dc.identifier.doi10.1016/j.jviromet.2004.01.005
dc.identifier.issn0166-0934
dc.identifier.lattes1923726000036625
dc.identifier.urihttp://hdl.handle.net/11449/7556
dc.identifier.wosWOS:000220770200006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Virological Methods
dc.relation.ispartofjcr1.756
dc.relation.ispartofsjr0,858
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectpiezoelectric biosensorpt
dc.subjecthepatitis C viruspt
dc.subjectRT-PCRpt
dc.titlePiezoelectric biosensors for real-time monitoring of hybridization and detection of hepatitis C virusen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes1923726000036625
unesp.author.lattes0173401604473200[2]
unesp.author.orcid0000-0003-2192-5312[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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Artigos - Química Analítica - IQ