Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?

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dc.contributor.authorTrevizan, Juliane Teramachi [UNESP]
dc.contributor.authorCarreira, Janaina Torres [UNESP]
dc.contributor.authorCarvalho, Isadora Resende [UNESP]
dc.contributor.authorKipper, Bruna Helena [UNESP]
dc.contributor.authorNagata, Walter Bertequine [UNESP]
dc.contributor.authorPerri, Silvia Helena Venturoli [UNESP]
dc.contributor.authorFranco Oliveira, Maria Emilia [UNESP]
dc.contributor.authorPierucci, Julia Cestari [UNESP]
dc.contributor.authorKoivisto, Marion Burkhardt de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:37:01Z
dc.date.available2018-12-11T17:37:01Z
dc.date.issued2018-08-01
dc.description.abstractThe aims of this study were to evaluate cryopreserved semen of Nellore bulls of different ages and verify whether sperm quality declines with advancing age and whether lipid peroxidation and DNA damage are involved in this process. For this purpose, 40 Nellore bulls were divided into three age groups: Young, aged 1.8–2 years (n = 9); Adult, aged 3.5–7.0 years (n = 19); and Seniors, aged 8.0–14.3 years (n = 12). Three ejaculates were collected from each bull, cryopreserved and evaluated for various parameters including membrane integrity, mitochondrial potential (FITC-PSA and JC1), lipid peroxidation (C-11BODIPY 581 / 591) and oxidative DNA damage (8OHdG) using flow cytometry. The thawed semen of senior bulls was characterized by a low percentage of motile sperm (33.7 ± 6.1%), higher damage to the plasma and acrosomal membrane (37.5 ± 9.8%), and low mitochondrial potential (29.1 ± 13.8%), as well as higher percentages of peroxidated cells (53.6 ± 12.2%) and DNA damage (44.1 ± 11.0%; P < 0.05). Lipid peroxidation was negatively correlated with motility (r = −0.35, P < 0.0002), average mitochondrial potential (r = −0.42; P < 0.0001) and showed a positive correlation with membrane injury and oxidative DNA damage (r = 039; P = 0.0003). Young bulls presented superior thawed sperm quality, possibly due to greater resistance to oxidative stress and, consequently, to cryopreservation. In conclusion, the sperm quality of bull semen declines with advancing age and is strongly associated with increased oxidative damage to both the plasma membrane and DNA.en
dc.description.affiliationDepartment of Medicine Surgery and Animal Reproduction School of Veterinary Medicine São Paulo State University – UNESP
dc.description.affiliationDepartment of Support Production and Animal Health School of Veterinary Medicine São Paulo State University – UNESP
dc.description.affiliationDepartment of Preventative Veterinary Medicine and Animal Reproduction School of Agricultural and Veterinary Sciences São Paulo State University – UNESP
dc.description.affiliationUnespDepartment of Medicine Surgery and Animal Reproduction School of Veterinary Medicine São Paulo State University – UNESP
dc.description.affiliationUnespDepartment of Support Production and Animal Health School of Veterinary Medicine São Paulo State University – UNESP
dc.description.affiliationUnespDepartment of Preventative Veterinary Medicine and Animal Reproduction School of Agricultural and Veterinary Sciences São Paulo State University – UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2011/16614-2
dc.description.sponsorshipIdFAPESP: 2011/22741-1
dc.format.extent8-15
dc.identifierhttp://dx.doi.org/10.1016/j.anireprosci.2018.04.071
dc.identifier.citationAnimal Reproduction Science, v. 195, p. 8-15.
dc.identifier.doi10.1016/j.anireprosci.2018.04.071
dc.identifier.file2-s2.0-85046826530.pdf
dc.identifier.issn0378-4320
dc.identifier.lattes3725971100777730
dc.identifier.scopus2-s2.0-85046826530
dc.identifier.urihttp://hdl.handle.net/11449/179853
dc.language.isoeng
dc.relation.ispartofAnimal Reproduction Science
dc.relation.ispartofsjr0,704
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAging
dc.subjectCryopreservation
dc.subjectReactive oxygen species (ROS)
dc.subjectSperm freezing
dc.titleDoes lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?en
dc.typeArtigo
unesp.author.lattes3725971100777730

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Artigos - Apoio, Produção e Saúde Animal - FMVA
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