Amphotericin B mediates killing in Cryptococcus neoformans through the induction of a strong oxidative burst

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dc.contributor.authorSangalli-Leite, Fernanda [UNESP]
dc.contributor.authorScorzoni, Liliana [UNESP]
dc.contributor.authorCecilia Mesa-Arango, Ana
dc.contributor.authorCasas, Celia
dc.contributor.authorHerrero, Enrique
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.authorLuis Rodriguez-Tudela, Juan
dc.contributor.authorCuenca-Estrella, Manuel
dc.contributor.authorZaragoza, Oscar
dc.contributor.institutionInst Salud Carlos III
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Antioquia
dc.contributor.institutionUniv Lleida
dc.date.accessioned2014-05-20T13:23:51Z
dc.date.available2014-05-20T13:23:51Z
dc.date.issued2011-05-01
dc.description.abstractWe studied the effects of Amphotericin B (AmB) on Cryptococcus neoformans using different viability methods (CFUs enumeration, XTT assay and propidium iodide permeability). After 1 h of incubation, there were no viable colonies when the cells were exposed to AmB concentrations >= 1 mg/L. In the same conditions, the cells did not become permeable to propidium iodide, a phenomenon that was not observed until 3 h of incubation. When viability was measured in parallel using XTT assay, a result consistent with the CFUs was obtained, although we also observed a paradoxical effect in which at high AmB concentrations, a higher XTT reduction was measured than at intermediate AmB concentrations. This paradoxical effect was not observed after 3 h of incubation with AmB, and lack of XTT reduction was observed at AmB concentrations higher than 1 mg/L. When stained with dihydrofluorescein, AmB induced a strong intracellular oxidative burst. Consistent with oxidative damage, AmB induced protein carbonylation. Our results indicate that in C. neoformans, Amphotericin B causes intracellular damage mediated through the production of free radicals before damage on the cell membrane, measured by propidium iodide uptake. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.en
dc.description.affiliationInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid 28220, Spain
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara SP, Lab Micol Clin, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv Antioquia, Grp Invest Dermatol, Medellin, Colombia
dc.description.affiliationUniv Lleida, IRBLleida, Dept Basic Med Sci, Lleida 25008, Spain
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara SP, Lab Micol Clin, BR-14801902 Araraquara, SP, Brazil
dc.description.sponsorshipSpanish Ministry of Science and Innovation
dc.description.sponsorshipCarolina Foundation
dc.description.sponsorshipIdSpanish MICINN: PCI2006-A7-0606
dc.description.sponsorshipIdSpanish MICINN: SAF2008-03761
dc.format.extent457-467
dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2011.01.015
dc.identifier.citationMicrobes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 5, p. 457-467, 2011.
dc.identifier.doi10.1016/j.micinf.2011.01.015
dc.identifier.issn1286-4579
dc.identifier.orcid0000-0002-8059-0826
dc.identifier.urihttp://hdl.handle.net/11449/7261
dc.identifier.wosWOS:000290084200005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMicrobes and Infection
dc.relation.ispartofjcr2.924
dc.relation.ispartofsjr1,205
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCryptococcus neoformansen
dc.subjectAmphotericin Ben
dc.subjectOxidative bursten
dc.subjectXTTen
dc.subjectPropidium iodideen
dc.titleAmphotericin B mediates killing in Cryptococcus neoformans through the induction of a strong oxidative bursten
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-8059-0826[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Análises Clínicas - FCFAR