Selection of X chromosome of buffaloes sperm with Percoll gradients

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dc.contributor.authorMota, A. V. [UNESP]
dc.contributor.authorOba, Eunice [UNESP]
dc.contributor.authorCastro, A. [UNESP]
dc.contributor.authorAraujo, G. H. M. [UNESP]
dc.contributor.authorStella, E. [UNESP]
dc.contributor.authorRamos, Alcides Amorim [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-02-26T17:18:36Z
dc.date.accessioned2014-05-20T13:40:43Z
dc.date.available2014-02-26T17:18:36Z
dc.date.available2014-05-20T13:40:43Z
dc.date.issued2007-01-01
dc.description.abstractThe aim of the present study was to evaluate the selection of X chromosome of buffaloes sperm with Percoll gradients. The stock solution of Percoll was prepared in the proportion of 1:11 (1 part of Percoll:11 parts of a solution containing KCl 1M, NaH(2)PO(4) 0.1M, NaCl 1.5M and sodium HEPES 23.8 g/ml). In order to prepare 9 different gradients were added to the stocked Percoll the A solution (glicine-yolk extender) in the following proportions: 90, 80, 72, 65, 57, 49, 34 and 25%. A sample of 0.7 ml of the fresh semen was deposited at 2 ml of Percoll 80% for the sperm wash. The precipitate was put in tube with 0.7 ml of each gradient. Then, the precipitated was washed in TES solution by centrifugation (500xg for 10 minutes), and collected again and diluted in TES solution to be freeze. The presence of the F body in the spermatozoa was observed in 58.7 +/- 5.4% of the control group and in 41.2 +/- 5.4% of the treated group (p<0.01). This result showed an increment of 17.55 of male sperm in the Percoll's group. The reduction of the centrifugation force did not improve the percentage of X sperm.en
dc.description.affiliationUNESP, FMVZ, Dept Anim Reprod & Radiol Vet, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUNESP, FMVZ, Dept Anim Prod, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, FMVZ, Dept Anim Reprod & Radiol Vet, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, FMVZ, Dept Anim Prod, BR-18618000 Botucatu, SP, Brazil
dc.format.extent807-809
dc.identifierhttp://dx.doi.org/10.4081%2Fijas.2007.s2.807
dc.identifierhttp://www.aspajournal.it/index.php/ijas/article/view/ijas.2007.s2.807
dc.identifier.citationItalian Journal of Animal Science. Pavia: Pagepress Publ, v. 6, p. 807-809, 2007.
dc.identifier.doi10.4081%2Fijas.2007.s2.807
dc.identifier.fileWOS000207598500022.pdf
dc.identifier.issn1594-4077
dc.identifier.lattes9224380586557664
dc.identifier.urihttp://hdl.handle.net/11449/14133
dc.identifier.wosWOS:000207598500022
dc.language.isoeng
dc.publisherPagepress Publ
dc.relation.ispartofItalian Journal of Animal Science
dc.relation.ispartofjcr0.990
dc.relation.ispartofsjr0,434
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectBuffaloespt
dc.subjectX chromosomept
dc.subjectSpermpt
dc.subjectPercoll gradientspt
dc.titleSelection of X chromosome of buffaloes sperm with Percoll gradientsen
dc.typeArtigo
dcterms.licensehttp://www.pagepress.org/open_access.html
dcterms.rightsHolderPagepress Publ
unesp.author.lattes9224380586557664
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Reprodução Animal e Radiologia Veterinária - FMVZ
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