Physical mapping of 18S rDNA and heterochromatin in species of family Lygaeidae (Hemiptera: Heteroptera)

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dc.contributor.authorBardella, V. B. [UNESP]
dc.contributor.authorSampaio, T. R.
dc.contributor.authorVenturelli, N. B.
dc.contributor.authorDias, A. L.
dc.contributor.authorGiuliano-Caetano, L.
dc.contributor.authorFernandes, J. A. M.
dc.contributor.authorRosa, R. da
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Fed Para
dc.date.accessioned2014-12-03T13:11:06Z
dc.date.available2014-12-03T13:11:06Z
dc.date.issued2014-01-01
dc.description.abstractAnalyses conducted using repetitive DNAs have contributed to better understanding the chromosome structure and evolution of several species of insects. There are few data on the organization, localization, and evolutionary behavior of repetitive DNA in the family Lygaeidae, especially in Brazilian species. To elucidate the physical mapping and evolutionary events that involve these sequences, we cytogenetically analyzed three species of Lygaeidae and found 2n (male) = 18 (16 + XY) for Oncopeltus femoralis; 2n (male) = 14 (12 + XY) for Ochrimnus sagax; and 2n (male) = 12 (10 + XY) for Lygaeus peruvianus. Each species showed different quantities of heterochromatin, which also showed variation in their molecular composition by fluorochrome staining. Amplification of the 18S rDNA generated a fragment of approximately 787 bp. The alignment of the consensus sequence with sequences from other species of Heteroptera deposited in the GenBank revealed a similarity of 98% with small differences. Fluorescent in situ hybridization with the 18S rDNA fragment revealed that this ribosomal gene was located in 1 autosomal pair at different positions in the three species. No cytogenetic data are available for these Brazilian species. The basal number and the possible chromosomal changes that occurred among the different species, as well as the evolution of these DNA sequences, are discussed.en
dc.description.affiliationUniv Estadual Londrina, Ctr Ciencias Biol, Dept Biol Geral, Lab Citogenet, Londrina, PR, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUniv Fed Para, Inst Ciencias Biol, Belem, PA, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent2186-2199
dc.identifierhttp://dx.doi.org/10.4238/2014.March.26.7
dc.identifier.citationGenetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 13, n. 1, p. 2186-2199, 2014.
dc.identifier.doi10.4238/2014.March.26.7
dc.identifier.fileWOS000334114800144.pdf
dc.identifier.issn1676-5680
dc.identifier.urihttp://hdl.handle.net/11449/112846
dc.identifier.wosWOS:000334114800144
dc.language.isoeng
dc.publisherFunpec-editora
dc.relation.ispartofGenetics and Molecular Research
dc.relation.ispartofsjr0,439
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectChromosome structureen
dc.subjectFluorescence in situ hybridizationen
dc.subjectHeterochromatinen
dc.subjectRibosomal genesen
dc.titlePhysical mapping of 18S rDNA and heterochromatin in species of family Lygaeidae (Hemiptera: Heteroptera)en
dc.typeArtigo
dcterms.rightsHolderFunpec-editora
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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