Effect of follicle size on mRNA expression in cumulus cells and oocytes of Bos indicus: an approach to identify marker genes for developmental competence

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dc.contributor.authorCaixeta, Ester Siqueira
dc.contributor.authorRipamonte, Paula [UNESP]
dc.contributor.authorFranco, Mauricio Machaim
dc.contributor.authorBuratini Junior, Jose [UNESP]
dc.contributor.authorNunes Dode, Margot Alves
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2014-05-20T13:49:33Z
dc.date.available2014-05-20T13:49:33Z
dc.date.issued2009-01-01
dc.description.abstractTo identify the genes related to oocyte competence, we quantified transcripts for candidate genes in oocytes (H1Foo, H2A, H3A, GHR, GDF9, BMP15, OOSP1) and cumulus cells (FSHR, EGFR, GHR, PTX3, IGFII) using the follicle size model to select oocytes of better developmental quality. Follicles were dissected and distributed into four groups according to diameter as follows: 1.0-3.0, 3.1-6.0, 6.1-8.0 and >= 8.1 mm. Cumulus-oocyte complexes (COCs) were released, classified morphologically, matured, fertilised and cultured in vitro or denuded for measurement of diameter and determination of gene expression. Denuded germinal vesicle oocytes and their cumulus cells were used for gene expression analysis by reverse transcription-polymerase chain reaction. The blastocyst rate was highest for oocytes recovered from follicles >6 mm in diameter. In the oocyte, expression of the H2A transcript only increased gradually according to follicle size, being greater (P < 0.05) in oocytes from follicles >= 8.1 mm in diameter than in oocytes from follicles <6.0 mm in diameter. In cumulus cells, expression of FSHR, EGFR and GHR mRNA increased with follicular size. In conclusion, we confirmed the importance of H2A for developmental competence and identified important genes in cumulus cells that may be associated with oocyte competence.en
dc.description.affiliationUniversidade de Brasilia (UnB), Fac Agron & Vet, BR-70910970 Brasilia, DF, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Fisiol, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Recursos Genet & Biotecnol, BR-70770900 Brasilia, DF, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Fisiol, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent655-664
dc.identifierhttp://dx.doi.org/10.1071/RD08201
dc.identifier.citationReproduction Fertility and Development. Collingwood: Csiro Publishing, v. 21, n. 5, p. 655-664, 2009.
dc.identifier.doi10.1071/RD08201
dc.identifier.issn1031-3613
dc.identifier.urihttp://hdl.handle.net/11449/17672
dc.identifier.wosWOS:000266406400005
dc.language.isoeng
dc.publisherCSIRO Publishing
dc.relation.ispartofReproduction, Fertility and Development
dc.relation.ispartofjcr2.105
dc.relation.ispartofsjr0,681
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbovineen
dc.subjectgene expressionen
dc.titleEffect of follicle size on mRNA expression in cumulus cells and oocytes of Bos indicus: an approach to identify marker genes for developmental competenceen
dc.typeArtigo
dcterms.licensehttp://www.publish.csiro.au/nid/36.htm
dcterms.rightsHolderCsiro Publishing
unesp.author.orcid0000-0003-4105-9079[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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