Crystallization and preliminary X-ray diffraction analysis of a glutathione S-transferase from Xylella fastidiosa

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dc.contributor.authorGarcia, Wanius
dc.contributor.authorTravensolo, Regiane F.
dc.contributor.authorRodrigues, Nathalia C.
dc.contributor.authorMuniz, Joao R. C.
dc.contributor.authorCaruso, Celia S.
dc.contributor.authorLemos, Eliana G. M. [UNESP]
dc.contributor.authorAraujo, Ana Paula U.
dc.contributor.authorCarrilho, Emanuel
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:17:44Z
dc.date.available2014-05-20T13:17:44Z
dc.date.issued2008-02-01
dc.description.abstractGlutathione S-transferases (GSTs) form a group of multifunctional isoenzymes that catalyze the glutathione-dependent conjugation and reduction reactions involved in the cellular detoxification of xenobiotic and endobiotic compounds. GST from Xylella fastidiosa (xfGST) was overexpressed in Escherichia coli and purified by conventional affinity chromatography. In this study, the crystallization and preliminary X-ray analysis of xfGST is described. The purified protein was crystallized by the vapour-diffusion method, producing crystals that belonged to the triclinic space group P1. The unit-cell parameters were a = 47.73, b = 87.73, c = 90.74 angstrom, alpha = 63.45, beta = 80.66, gamma = 94.55 degrees. xfGST crystals diffracted to 2.23 angstrom resolution on a rotating-anode X-ray source.en
dc.description.affiliationUniv São Paulo, Lab Biofis Mol Sergio Mascarenhas, Inst Fis Sao Carlos, Sao Carlos, SP, Brazil
dc.description.affiliationUniv São Paulo, Grp Bioanalit Microfabricacao & Separacoes, Inst Quim Sao Carlos, Sao Carlos, SP, Brazil
dc.description.affiliationUNESP, Lab Bioquim Microrganismos & Plantas, Dept Technol, Jaboticabal, Brazil
dc.description.affiliationUnespUNESP, Lab Bioquim Microrganismos & Plantas, Dept Technol, Jaboticabal, Brazil
dc.format.extent85-87
dc.identifierhttp://dx.doi.org/10.1107/S174430910706825X
dc.identifier.citationActa Crystallographica Section F-structural Biology and Crystallization Communications. Oxford: Blackwell Publishing, v. 64, p. 85-87, 2008.
dc.identifier.doi10.1107/S174430910706825X
dc.identifier.fileWOS000252817400006.pdf
dc.identifier.issn1744-3091
dc.identifier.urihttp://hdl.handle.net/11449/4092
dc.identifier.wosWOS:000252817400006
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleCrystallization and preliminary X-ray diffraction analysis of a glutathione S-transferase from Xylella fastidiosaen
dc.typeArtigo
dcterms.licensehttp://journals.iucr.org/services/copyrightpolicy.html
dcterms.rightsHolderBlackwell Publishing
unesp.author.orcid0000-0001-7351-8220[8]
unesp.author.orcid0000-0001-5455-084X[7]
unesp.author.orcid0000-0003-2731-3966[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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