Improved esthetic efficacy and reduced cytotoxicity are achieved with a violet LED irradiation of manganese oxide-enriched bleaching gels

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dc.contributor.authorDias, Marlon Ferreira [UNESP]
dc.contributor.authorMartins, Beatriz Voss [UNESP]
dc.contributor.authorde Oliveira Ribeiro, Rafael Antonio [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorde Souza Costa, Carlos Alberto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T16:00:58Z
dc.date.available2023-07-29T16:00:58Z
dc.date.issued2023-12-01
dc.description.abstractGels with high concentrations of hydrogen peroxide (H2O2) have been associated with cytotoxicity and consequent post-bleaching tooth sensitivity. This study assessed the bleaching efficacy (BE) and cytotoxicity (CT) of bleaching gels with low concentrations of H2O2 containing manganese oxide (MnO2) and photocatalyzed with violet LED (LEDv). The following groups were established: G1: no treatment (negative control, NC); G2: 35% H2O2 (positive control, PC); G3: LEDv; G4: 10% H2O2; G5: 6% H2O2; G6: 10% H2O2 + MnO2 + LEDv; G7: 6% H2O2 + MnO2 + LEDv. To analyze BE, standardized enamel/dentin discs (E/DDs) were subjected to the bleaching procedures for 45 min (1 session). The color change was determined before and after performing the bleaching protocols (ΔE00; ΔWI). To analyze CT, the E/DDs were adapted to artificial pulp chambers, and the extracts (culture medium + diffused gel components) were applied to cultured odontoblast-like MDPC-23 cells. Then, the cells were assessed concerning their viability (VB), oxidative stress (OxS), and Live/Dead. The amount of H2O2 diffused was also determined (ANOVA/Tukey; p < 0.05). Cell viability decreased in all bleached groups compared to G1 (NC; p < 0.05). The cells in G6 and G7 presented higher viability than in G2, G4, and G5 (p < 0.05). The BE in G7 was similar to G2 (PC; p < 0.05). The lowest OxS and H2O2 diffusion values were found in G6 and G7, compared to the other bleached groups (G2, G4, and G5; p < 0.05). The 6% H2O2 bleaching gel (G7) submitted to both methods of catalysis (MnO2 + LEDv) caused only a mild cytotoxicity and maintained the excellent esthetic outcome promoted by in-office conventional tooth bleaching.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), Rua Humaitá
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), Rua Humaitá
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdFAPESP: 2020/09095-8
dc.description.sponsorshipIdFAPESP: 2021/01184-4
dc.description.sponsorshipIdCNPq: 302047/2019-0
dc.description.sponsorshipIdCNPq: 408721/2018-9
dc.identifierhttp://dx.doi.org/10.1007/s10103-022-03688-9
dc.identifier.citationLasers in Medical Science, v. 38, n. 1, 2023.
dc.identifier.doi10.1007/s10103-022-03688-9
dc.identifier.issn1435-604X
dc.identifier.issn0268-8921
dc.identifier.scopus2-s2.0-85144322888
dc.identifier.urihttp://hdl.handle.net/11449/249488
dc.language.isoeng
dc.relation.ispartofLasers in Medical Science
dc.sourceScopus
dc.subjectCell culture
dc.subjectDental materials
dc.subjectTooth bleaching
dc.subjectToxicity
dc.titleImproved esthetic efficacy and reduced cytotoxicity are achieved with a violet LED irradiation of manganese oxide-enriched bleaching gelsen
dc.typeArtigo

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