D2 region of the 28S RNA gene: A too-conserved fragment for inferences on phylogeny of South American triatomines

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dc.contributor.authorGuerra, Ana Letícia [UNESP]
dc.contributor.authorAlevi, Kaio Cesar Chaboli [UNESP]
dc.contributor.authorBanho, Cecília Artico [UNESP]
dc.contributor.authorDe Oliveira, Jader [UNESP]
dc.contributor.authorDa Rosa, João Aristeu [UNESP]
dc.contributor.authorDe Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:05:24Z
dc.date.available2018-12-11T17:05:24Z
dc.date.issued2016-09-01
dc.description.abstractThe brasiliensis complex is composed of five triatomine species, and different approaches suggest that Triatoma lenti and Triatoma petrochiae may be the new members. Therefore, this study sought to analyze the phylogenetic relationships within this complex by means of the D2 region of the 28S RNA gene, and to analyze the degree of polymorphism and phylogenetic significance of this gene for South American triatomines. Phylogenetic analysis by using sequence fragments of the D2 domain did not allow to perform phylogenetic inferences on species within the brasiliensis complex, because the gene alignment composed of a matrix with 37 specimens exhibited only two variable sites along the 567 base pairs used. Furthermore, if all South American species are included, only four variable sites were detected, reflecting the high degree of gene conservation. Therefore, we do not recommend the use of this gene for phylogenetic reconstruction for this group of Chagas disease vectors.en
dc.description.affiliationDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista
dc.description.affiliationInstituto de Biociências Letras e Ciências Exatas (IBILCE-UNESP), Rua Cristovão Colombo, 2265, Jardim Nazareth
dc.description.affiliationUnespDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista
dc.description.affiliationUnespInstituto de Biociências Letras e Ciências Exatas (IBILCE-UNESP), Rua Cristovão Colombo, 2265, Jardim Nazareth
dc.format.extent610-613
dc.identifierhttp://dx.doi.org/10.4269/ajtmh.15-0747
dc.identifier.citationAmerican Journal of Tropical Medicine and Hygiene, v. 95, n. 3, p. 610-613, 2016.
dc.identifier.doi10.4269/ajtmh.15-0747
dc.identifier.issn0002-9637
dc.identifier.scopus2-s2.0-84984817968
dc.identifier.urihttp://hdl.handle.net/11449/173429
dc.language.isoeng
dc.relation.ispartofAmerican Journal of Tropical Medicine and Hygiene
dc.relation.ispartofsjr1,430
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.titleD2 region of the 28S RNA gene: A too-conserved fragment for inferences on phylogeny of South American triatominesen
dc.typeArtigo

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