Role of annexin 1 gene expression in mouse craniofacial bone development
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2007-07-01
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BACKGROUND: Annexin 1 is a 37-kDa protein that has complex intra- and extracellular effects. To discover whether the absence of this protein alters bone development, we monitored this event in the annexin-A1 null mice in comparison with littermate wild-type controls. METHODS: Radiographic and densitometry methods were used for the assessment of bone in annexin-A1 null mice at a gross level. We used whole-skeleton staining, histological analysis, and Western blotting techniques to monitor changes at the tissue and cellular levels. RESULTS: There were no gross differences in the appendicular skeleton between the genotypes, but an anomalous development of the skull was observed in the annexin-A1 null mice. This was characterized in the newborn annexin-A1 null animals by a delayed intramembranous ossification of the skull, incomplete fusion of the interfrontal suture and palatine bone, and the presence of an abnormal suture structure. The annexin-A1 gene was shown to be active in osteocytes during this phase and COX-2 was abundantly expressed in cartilage and bone taken from annexin-A1 null mice. CONCLUSIONS: Expression of the annexin-A1 gene is important for the normal development of the skull in mice, possibly through the regulation of osteoblast differentiation and a secondary effect on the expression of components of the cPLA2-COX-2 system. © 2007 Wiley-Liss, Inc.
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Annexin 1 , Bone , Cyclooxygenase-2 , Cytosolic phospholipase A2 , Osteoblast , annexin , annexin 1 , cyclooxygenase 2 , phospholipase A2 , protein , unclassified drug , adolescent , animal tissue , annexin a1 gene , bone density , controlled study , craniofacial development , female , gene , gene activation , histology , immunohistochemistry , male , mouse , newborn , nonhuman , ossification , osteocyte , priority journal , protein expression , skull suture , Western blotting , Animals , Animals, Newborn , Annexin A1 , Bone and Bones , Bone Density , Bone Development , Craniofacial Abnormalities , Cyclooxygenase 2 , Female , Gene Expression , Homozygote , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Osteogenesis , Phospholipases A , Animalia , Mus
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Birth Defects Research Part A - Clinical and Molecular Teratology, v. 79, n. 7, p. 524-532, 2007.