Genetic diversity of babesia bovis studied longitudinally under natural transmission conditions in calves in the state of rio de janeiro, brazil

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dc.contributor.authorMatos, Carlos António [UNESP]
dc.contributor.authorSilva, Jenevaldo Barbosa da [UNESP]
dc.contributor.authorGonçalves, Luiz Ricardo [UNESP]
dc.contributor.authorMendes, Natalia Serra [UNESP]
dc.contributor.authorAlvarez, Dasiel Obregón
dc.contributor.authorAndré, Marcos Rogério [UNESP]
dc.contributor.authorMachado, Rosangela Zacarias [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionDirecção de Ciências Animais
dc.contributor.institutionUniversidad Agraria de La Habana
dc.date.accessioned2021-06-25T10:45:32Z
dc.date.available2021-06-25T10:45:32Z
dc.date.issued2020-01-01
dc.description.abstractSerum and DNA samples from 15 naturally infected calves in Seropédica, Brazil, were obtained quarterly from birth to 12 months of age, in order to longitudinally evaluate their humoral immune response against Babesia bovis and the merozoite surface antigen diversity of B. bovis. Anti-B. bovis IgG antibodies were detected by an indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA). Using DNA amplification, sequencing and phylogenetic analysis, the genetic diversity of B. bovis was assessed based on the genes that encode merozoite surface antigens (MSA-1, MSA-2b and MSA-2c). The serological results demonstrated that up to six months of age, all the calves developed active immunity against B. bovis. Among the 75 DNA samples evaluated, 0, 3 and 5 sequences of the msa-1, msa-2b and msa-2c genes were obtained, respectively. The present study demonstrated that the msa-2b and msa-2c gene sequences amplified from blood DNA of B. bovis-positive calves were genetically diversified. These data emphasize the importance of conducting deeper studies on the genetic diversity of B. bovis in Brazil, in order to design diagnostic antigens and vaccines in the future.en
dc.description.affiliationDepartamento de Patologia Reprodução e Saúde Única Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP)
dc.description.affiliationDirecção de Ciências Animais
dc.description.affiliationUniversidad Agraria de La Habana
dc.description.affiliationUnespDepartamento de Patologia Reprodução e Saúde Única Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipUniversidade Estadual Paulista
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012 213714
dc.description.sponsorshipIdCNPq: 302420/2017-7
dc.format.extent1-11
dc.identifierhttp://dx.doi.org/10.1590/S1984-29612020089
dc.identifier.citationRevista Brasileira de Parasitologia Veterinaria, v. 29, n. 4, p. 1-11, 2020.
dc.identifier.doi10.1590/S1984-29612020089
dc.identifier.fileS1984-29612020000400312.pdf
dc.identifier.issn1984-2961
dc.identifier.issn0103-846X
dc.identifier.scieloS1984-29612020000400312
dc.identifier.scopus2-s2.0-85096817146
dc.identifier.urihttp://hdl.handle.net/11449/206890
dc.language.isoeng
dc.relation.ispartofRevista Brasileira de Parasitologia Veterinaria
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBabesia bovis
dc.subjectBovine babesiosis
dc.subjectGenetic diversity
dc.subjectMSA
dc.subjectSerology
dc.titleGenetic diversity of babesia bovis studied longitudinally under natural transmission conditions in calves in the state of rio de janeiro, brazilen
dc.titleDiversidade genética de babesia bovis estudada longitudinalmente em condições de transmissão natural entre bezerros no estado do rio de janeiro, brasilpt
dc.typeArtigo

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Artigos - Patologia Veterinária - FCAV