B chromosome in the beetle Coprophanaeus cyanescens (Scarabaeidae): emphasis in the organization of repetitive DNA sequences

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dc.contributor.authorde Oliveira, Sarah Gomes [UNESP]
dc.contributor.authorde Moura, Rita Cassia
dc.contributor.authorMartins, Cesar [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUPF Pernambuco State Univ
dc.date.accessioned2014-05-20T13:52:29Z
dc.date.available2014-05-20T13:52:29Z
dc.date.issued2012-11-06
dc.description.abstractBackground: To contribute to the knowledge of coleopteran cytogenetics, especially with respect to the genomic content of B chromosomes, we analyzed the composition and organization of repetitive DNA sequences in the Coprophanaeus cyanescens karyotype. We used conventional staining and the application of fluorescence in situ hybridization (FISH) mapping using as probes C(0)t-1 DNA fraction, the 18S and 5S rRNA genes, and the LOA-like non-LTR transposable element (TE).Results: The conventional analysis detected 3 individuals (among 50 analyzed) carrying one small metacentric and mitotically unstable B chromosome. The FISH analysis revealed a pericentromeric block of C(0)t-1 DNA in the B chromosome but no 18S or 5S rDNA clusters in this extra element. Using the LOA-like TE probe, the FISH analysis revealed large pericentromeric blocks in eight autosomal bivalents and in the B chromosome, and a pericentromeric block extending to the short arm in one autosomal pair. No positive hybridization signal was observed for the LOA-like element in the sex chromosomes.Conclusions: The results indicate that the origin of the B chromosome is associated with the autosomal elements, as demonstrated by the hybridization with C(0)t-1 DNA and the LOA-like TE. The present study is the first report on the cytogenetic mapping of a TE in coleopteran chromosomes. These TEs could have been involved in the origin and evolution of the B chromosome in C. cyanescens.en
dc.description.affiliationUNESP São Paulo State Univ, Dept Morphol, Biosci Inst, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUPF Pernambuco State Univ, Inst Biol Sci, Dept Biol, BR-50100130 Recife, PE, Brazil
dc.description.affiliationUnespUNESP São Paulo State Univ, Dept Morphol, Biosci Inst, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent6
dc.identifierhttp://dx.doi.org/10.1186/1471-2156-13-96
dc.identifier.citationBmc Genetics. London: Biomed Central Ltd., v. 13, p. 6, 2012.
dc.identifier.doi10.1186/1471-2156-13-96
dc.identifier.fileWOS000312340700001.pdf
dc.identifier.issn1471-2156
dc.identifier.lattes8858800699425352
dc.identifier.orcid0000-0003-3534-974X
dc.identifier.urihttp://hdl.handle.net/11449/18746
dc.identifier.wosWOS:000312340700001
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofBMC Genetics
dc.relation.ispartofjcr2.469
dc.relation.ispartofsjr1,160
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectChromosomal rearrangementsen
dc.subjectHeterochromatinen
dc.subjectMultigene familiesen
dc.subjectSupernumerary chromosomeen
dc.subjectTransposable elementsen
dc.titleB chromosome in the beetle Coprophanaeus cyanescens (Scarabaeidae): emphasis in the organization of repetitive DNA sequencesen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
unesp.author.lattes8858800699425352[3]
unesp.author.orcid0000-0003-3534-974X[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Morfologia - IBB