Subcellular localization of proteins labeled with GFP in Xanthomonas citri ssp citri: targeting the division septum

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dc.contributor.authorMartins, Paula M. M. [UNESP]
dc.contributor.authorLau, Ivy F. [UNESP]
dc.contributor.authorBacci Junior, Mauricio [UNESP]
dc.contributor.authorBelasque, Jose
dc.contributor.authordo Amaral, Alexandre M.
dc.contributor.authorTaboga, Sebastiao R. [UNESP]
dc.contributor.authorFerreira, Henrique [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFundo Defesa Citricultura
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2014-05-20T14:00:51Z
dc.date.available2014-05-20T14:00:51Z
dc.date.issued2010-09-01
dc.description.abstractXanthomonas citri ssp. citri (Xac) is the causal agent of citrus canker, an economically important disease that affects citrus worldwide. To initiate the characterization of essential biological processes of Xac, we constructed integrative plasmids for the ectopic expression of green fluorescent protein (GFP)-labeled proteins within this bacterium. Here, we show that the disruption of the alpha-amylase gene (amy), the site of plasmid integration into the bacterial chromosome, does not alter its pathogenesis while abolishing completely the ability of Xac to degrade starch. Furthermore, our GFP expression system was used to characterize ORF XAC3408, a hypothetical protein encoded by Xac that shares significant homology to the FtsZ-stabilizing factor ZapA from Bacillus subtilis (ZapABsu). GFP-XAC3408 expressed in Xac exhibited a septal localization pattern typical of GFP-ZapABsu, which indicates that XAC3408 is the Xac orthologue of the cell division protein ZapABsu. The results demonstrate the potential of GFP labeling for protein functional characterizations in Xac, and, in addition, the Xac mutant strain labeled at the septum constitutes a biological model for the exploration of antibacterial compounds able to inhibit cell division in this plant pathogen.en
dc.description.affiliationUniv Estadual Paulista, Dept Ciencias Biol, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Ctr Estudos Insetos Sociais, Inst Biociencias, Rio Claro, SP, Brazil
dc.description.affiliationFundo Defesa Citricultura, Araraquara, SP, Brazil
dc.description.affiliationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Recursos Genet & Biotecnol, Brasilia, DF, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Biol, Sao Jose do Rio Preto, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Ciencias Biol, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Ctr Estudos Insetos Sociais, Inst Biociencias, Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Biol, Sao Jose do Rio Preto, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 06/59494-9
dc.description.sponsorshipIdFAPESP: 04/09173-6
dc.format.extent76-83
dc.identifierhttp://dx.doi.org/10.1111/j.1574-6968.2010.02047.x
dc.identifier.citationFems Microbiology Letters. Malden: Wiley-blackwell, v. 310, n. 1, p. 76-83, 2010.
dc.identifier.doi10.1111/j.1574-6968.2010.02047.x
dc.identifier.issn0378-1097
dc.identifier.lattes3776345573864268
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.urihttp://hdl.handle.net/11449/21493
dc.identifier.wosWOS:000280633200011
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofFEMS Microbiology Letters
dc.relation.ispartofjcr1.735
dc.relation.ispartofsjr0,790
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectcitrus cankeren
dc.subjectcell divisionen
dc.subjectalpha-amylaseen
dc.titleSubcellular localization of proteins labeled with GFP in Xanthomonas citri ssp citri: targeting the division septumen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.lattes3776345573864268
unesp.author.orcid0000-0001-6059-2173[4]
unesp.author.orcid0000-0002-5619-1411[3]
unesp.author.orcid0000-0002-0970-4288[6]
unesp.author.orcid0000-0002-9183-9420[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Ciências Biológicas - FCFAR
Artigos - Biologia - IBILCE
Rio Claro - CEIS - Centro de Estudos de Insetos Sociais