Comparison of Brucella agar, CITA and Farrel media for selective isolation of Brucella abortus from semen of bovine bulls
dc.contributor.author | Nardi Júnior, Geraldo de | |
dc.contributor.author | Megid, Jane [UNESP] | |
dc.contributor.author | Vicente, Acácia Ferreira [UNESP] | |
dc.contributor.author | Listoni, Fernando José Paganini [UNESP] | |
dc.contributor.author | Monteiro, Fábio Morato | |
dc.contributor.author | Lara, Gustavo Henrique Batista [UNESP] | |
dc.contributor.author | Motta, Rodrigo Garcia [UNESP] | |
dc.contributor.author | Chacur, Marcelo George Mungai | |
dc.contributor.author | Ribeiro, Marcio Garcia [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade do Oeste Paulista (UNOESTE) | |
dc.contributor.institution | Agencia Paulista de Tecnologia em Agronegocio (APTA) | |
dc.date.accessioned | 2016-04-01T18:43:56Z | |
dc.date.available | 2016-04-01T18:43:56Z | |
dc.date.issued | 2015 | |
dc.description.abstract | Brucellosis remains as a public health concern worldwide. In domestic animals, the disease is characterized by reproductive disorders in male and female. Besides extensive use of serological tests and recent development of molecular biology techniques, microbiological culture of Brucella species is yet considered a “gold standard” method for diagnosis. Here, semen of 335 bovine bulls was subjected simultaneously to microbiological culture in Brucella agar, Farrell media, and CITA media to evaluate comparatively the best selective media for isolation of Brucella sp. Among all 335 samples, B. abortus B19 strain was isolated from semen of five (1.49%) bulls using the three selective media. However, Farrell media was considered the best selective media for microbiological diagnosis, because of allowed isolation of B. abortus B19 strain from bull semen without bacterial commensal or fungal contamination of plates. | en |
dc.description.affiliation | Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Botucatu, Departamento de Higiene Veterinária e Saúde Pública, Distrito Rubião Júnior, CEP 18618-000, SP, Brasil | |
dc.description.affiliationUnesp | Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Botucatu, Departamento de Higiene Veterinária e Saúde Pública, Distrito Rubião Júnior, CEP 18618-000, SP, Brasil | |
dc.format.extent | 617-620 | |
dc.identifier | http://dx.doi.org/10.5897/AJMR2014.7252 | |
dc.identifier.citation | African Journal of Microbiology Research, v. 9, n. 9, p. 617-620, 2015. | |
dc.identifier.doi | 10.5897/AJMR2014.7252 | |
dc.identifier.issn | 1996-0808 | |
dc.identifier.lattes | 0475227077170072 | |
dc.identifier.lattes | 2209124317273797 | |
dc.identifier.lattes | 6968547626233995 | |
dc.identifier.lattes | 2209124317273797 | |
dc.identifier.uri | http://hdl.handle.net/11449/137046 | |
dc.language.iso | eng | |
dc.relation.ispartof | African Journal of Microbiology Research | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Currículo Lattes | |
dc.subject | Brucella agar | en |
dc.subject | Farrell media | en |
dc.subject | CITA media | en |
dc.subject | Bovine bulls | en |
dc.subject | Semen | en |
dc.title | Comparison of Brucella agar, CITA and Farrel media for selective isolation of Brucella abortus from semen of bovine bulls | en |
dc.type | Artigo | |
unesp.author.lattes | 0475227077170072 | |
unesp.author.lattes | 2209124317273797 | |
unesp.author.lattes | 6968547626233995 | |
unesp.author.lattes | 2209124317273797 | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatu | pt |
unesp.department | Higiene Veterinária e Saúde Pública | pt |