Comparison of Brucella agar, CITA and Farrel media for selective isolation of Brucella abortus from semen of bovine bulls

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dc.contributor.authorNardi Júnior, Geraldo de
dc.contributor.authorMegid, Jane [UNESP]
dc.contributor.authorVicente, Acácia Ferreira [UNESP]
dc.contributor.authorListoni, Fernando José Paganini [UNESP]
dc.contributor.authorMonteiro, Fábio Morato
dc.contributor.authorLara, Gustavo Henrique Batista [UNESP]
dc.contributor.authorMotta, Rodrigo Garcia [UNESP]
dc.contributor.authorChacur, Marcelo George Mungai
dc.contributor.authorRibeiro, Marcio Garcia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade do Oeste Paulista (UNOESTE)
dc.contributor.institutionAgencia Paulista de Tecnologia em Agronegocio (APTA)
dc.date.accessioned2016-04-01T18:43:56Z
dc.date.available2016-04-01T18:43:56Z
dc.date.issued2015
dc.description.abstractBrucellosis remains as a public health concern worldwide. In domestic animals, the disease is characterized by reproductive disorders in male and female. Besides extensive use of serological tests and recent development of molecular biology techniques, microbiological culture of Brucella species is yet considered a “gold standard” method for diagnosis. Here, semen of 335 bovine bulls was subjected simultaneously to microbiological culture in Brucella agar, Farrell media, and CITA media to evaluate comparatively the best selective media for isolation of Brucella sp. Among all 335 samples, B. abortus B19 strain was isolated from semen of five (1.49%) bulls using the three selective media. However, Farrell media was considered the best selective media for microbiological diagnosis, because of allowed isolation of B. abortus B19 strain from bull semen without bacterial commensal or fungal contamination of plates.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Botucatu, Departamento de Higiene Veterinária e Saúde Pública, Distrito Rubião Júnior, CEP 18618-000, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Botucatu, Departamento de Higiene Veterinária e Saúde Pública, Distrito Rubião Júnior, CEP 18618-000, SP, Brasil
dc.format.extent617-620
dc.identifierhttp://dx.doi.org/10.5897/AJMR2014.7252
dc.identifier.citationAfrican Journal of Microbiology Research, v. 9, n. 9, p. 617-620, 2015.
dc.identifier.doi10.5897/AJMR2014.7252
dc.identifier.issn1996-0808
dc.identifier.lattes0475227077170072
dc.identifier.lattes2209124317273797
dc.identifier.lattes6968547626233995
dc.identifier.lattes2209124317273797
dc.identifier.urihttp://hdl.handle.net/11449/137046
dc.language.isoeng
dc.relation.ispartofAfrican Journal of Microbiology Research
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectBrucella agaren
dc.subjectFarrell mediaen
dc.subjectCITA mediaen
dc.subjectBovine bullsen
dc.subjectSemenen
dc.titleComparison of Brucella agar, CITA and Farrel media for selective isolation of Brucella abortus from semen of bovine bullsen
dc.typeArtigo
unesp.author.lattes0475227077170072
unesp.author.lattes2209124317273797
unesp.author.lattes6968547626233995
unesp.author.lattes2209124317273797
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentHigiene Veterinária e Saúde Públicapt

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