Vitamin B12-induced spermatogenesis recovery in cimetidine-treated rats: Effect on the spermatogonia number and sperm concentration

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dc.contributor.authorBeltrame, Flávia I.
dc.contributor.authorSasso-Cerri, Estela [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:14:05Z
dc.date.available2018-12-11T17:14:05Z
dc.date.issued2017-09-01
dc.description.abstractThe H2-receptor antagonist cimetidine is an antiulcer drug also used for the treatment of cancer due to its antiangiogenic effect. However, this drug has caused structural changes in the seminiferous tubules. Vitamin B12 has been used as a therapeutic agent for the treatment of male infertility. The supplementation of rats with Vitamin B12 during cimetidine treatment has recovered the damaged seminiferous tubules, but how this vitamin restores the seminiferous epithelium has not been clarified. In this study, we evaluated whether Vitamin B12 improves the number of spermatogonia, spermatocytes, and sperm concentration in cimetidine-treated rats. Adult male rats were treated for 50 days as follows: cimetidine group received 100 mg kg-1 b.w. of cimetidine, cimetidine-B12 group received cimetidine and 3 μg of Vitamin B12-hydroxocobalamin, B12 group received only 3 μg of vitamin, and control group received saline. Sperm concentration was calculated and historesin-embedded testes sections were used for the quantitative analyses of spermatogonia (A; In/B) and spermatocytes. TUNEL method and PCNA immunofluorescence were performed. Cimetidine caused a significant reduction in sperm concentration. TUNEL-positive spermatogonia and spermatocytes were correlated to a significant reduction in the number of these cells. In cimetidine-B12 group, sperm concentration was higher than cimetidine group and a significant increase in the number of spermatogonia (stages II-VI) was correlated to a high incidence of PCNA-immunolabeled spermatogonia and spermatocytes. The results show that the supplementation of rats with Vitamin B12 during cimetidine treatment increases sperm concentration and exerts a potential effect in the recovery of spermatogonia and spermatocytes.en
dc.description.affiliationDepartment of Morphology and Genetics Federal University of São Paulo (UNIFESP/EPM)
dc.description.affiliationDepartment of Morphology Laboratory of Histology and Embryology Dental School-São Paulo State University (UNESP/FOAr)
dc.description.affiliationUnespDepartment of Morphology Laboratory of Histology and Embryology Dental School-São Paulo State University (UNESP/FOAr)
dc.format.extent567-572
dc.identifierhttp://dx.doi.org/10.4103/1008-682X.182397
dc.identifier.citationAsian Journal of Andrology, v. 19, n. 5, p. 567-572, 2017.
dc.identifier.doi10.4103/1008-682X.182397
dc.identifier.file2-s2.0-85027886980.pdf
dc.identifier.issn1745-7262
dc.identifier.issn1008-682X
dc.identifier.scopus2-s2.0-85027886980
dc.identifier.urihttp://hdl.handle.net/11449/175067
dc.language.isoeng
dc.relation.ispartofAsian Journal of Andrology
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCimetidine
dc.subjectFertility
dc.subjectSpermatogenesis
dc.subjectTestis
dc.subjectVitamin B12
dc.titleVitamin B12-induced spermatogenesis recovery in cimetidine-treated rats: Effect on the spermatogonia number and sperm concentrationen
dc.typeArtigo

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