Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health

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dc.contributor.authorBorsanelli, Ana C. [UNESP]
dc.contributor.authorLappin, David F.
dc.contributor.authorViora, Lorenzo
dc.contributor.authorKing, George
dc.contributor.authorBennett, David
dc.contributor.authorDutra, Iveraldo S. [UNESP]
dc.contributor.authorRiggio, Marcello P.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Glasgow
dc.date.accessioned2018-11-29T18:48:45Z
dc.date.available2018-11-29T18:48:45Z
dc.date.issued2018-06-01
dc.description.abstractBovine periodontitis is a progressive and purulent infection associated with an anaerobic subgingival biofilm, which induces irreversible damage to the dentition of affected animals. The aetiopathogenesis of the disease is unclear and treatment and control of the disease process in cattle are almost unknown. The aim of this study was to investigate the innate immune response by quantifying expression of Toll-like receptor (TLR) and cytokine genes in gingival tissue samples from cattle with and without periodontitis. Postmortem biopsies of gingival tissues were collected from 20 cattle with periodontitis and 20 cattle with no clinical signs of periodontal lesions. Tissue expression of TLR2, TLR4, TNF-alpha, IFN-gamma, IL-1 beta and IL-4 genes were determined using quantitative real-time PCR. Statistically significant increases in mRNA levels encoding TLR2 (p = 0.025), TLR4 (p = 0.037), TNF-alpha (p = 0.025), IFN-gamma (p = 0.014), IL-1 beta (p < 0.001) and IL-4 (p = 0.014) were observed in animals with periodontitis when compared to periodontally healthy animals. Increased levels of TLRs and inflammatory cytokines in periodontal tissue indicate an induction of the innate immune response of cattle and suggest that a substantial microbial challenge may be involved in the aetiopathogenesis of bovine periodontitis.en
dc.description.affiliationUniv Estadual Paulista, Fac Vet Med, Dept Anim Hlth & Prod, Sao Paulo, Brazil
dc.description.affiliationUniv Glasgow, Sch Dent, Oral Sci Res Grp, Glasgow, Lanark, Scotland
dc.description.affiliationUniv Glasgow, Sch Vet Med, Glasgow, Lanark, Scotland
dc.description.affiliationUnespUniv Estadual Paulista, Fac Vet Med, Dept Anim Hlth & Prod, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: FAPESP2015/06917-9
dc.format.extent439-443
dc.identifierhttp://dx.doi.org/10.1016/j.rvsc.2018.04.008
dc.identifier.citationResearch In Veterinary Science. Oxford: Elsevier Sci Ltd, v. 118, p. 439-443, 2018.
dc.identifier.doi10.1016/j.rvsc.2018.04.008
dc.identifier.fileWOS000435059400057.pdf
dc.identifier.issn0034-5288
dc.identifier.urihttp://hdl.handle.net/11449/166196
dc.identifier.wosWOS:000435059400057
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofResearch In Veterinary Science
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectBovine
dc.subjectPeriodontitis
dc.subjectToll-like receptors
dc.subjectCytokines
dc.subjectInnate immune response
dc.titleEvaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral healthen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

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