Punica granatum L. (Pomegranate) extract: In vivo study of antimicrobial activity against Porphyromonas gingivalis in galleria mellonella model

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dc.contributor.authorAparecida Procópio Gomes, Livia [UNESP]
dc.contributor.authorAlves Figueiredo, Lívia Mara [UNESP]
dc.contributor.authorLuiza Do Rosário Palma, Ana [UNESP]
dc.contributor.authorCorrêa Geraldo, Barbara Maria [UNESP]
dc.contributor.authorIsler Castro, Kelly Cristine [UNESP]
dc.contributor.authorRuano De Oliveira Fugisaki, Luciana [UNESP]
dc.contributor.authorOlavo Cardoso Jorge, Antônio [UNESP]
dc.contributor.authorDias De Oliveira, Luciane [UNESP]
dc.contributor.authorCampos Junqueira, Juliana [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:06:09Z
dc.date.available2018-12-11T17:06:09Z
dc.date.issued2016-01-01
dc.description.abstractDue to the increase of bacterial resistance, medicinal alternatives are being explored. Punica granatum L. is an effective herbal extract with broad spectrum of action and bactericidal, antifungal, anthelmintic potential and being able to modulate the immune response. The aim was to evaluate the antimicrobial activity of pomegranate glycolic extract (PGE) against the periodontal pathogen Porphyromonas gingivalis by using Galleria mellonella as in vivo model. Fifteen larvae were used per group. Injection of high concentration (200, 100, and 25 mg/mL) of PGE showed a toxic effect, leading them to death. A suspension of P. gingivalis (106 cells/mL) was inoculated in the left last proleg and PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) were injected into the right proleg. The larvae were then kept at 37°C under the dark. Injection of PGE at any dose statistically improved larvae survival rates. The data were analysed (log-rank test, Mantel-Cox, P < 0.05) and showed that all concentrations of PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) presented higher larval survival rates, with significant statistical difference in relation to control group (P. gingivalis). In conclusion, the PGE had antimicrobial action against P. gingivalis in vivo model using G. mellonella.en
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis Laboratory of Microbiology and Immunology Institute of Science and Technology Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis Laboratory of Microbiology and Immunology Institute of Science and Technology Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777
dc.identifierhttp://dx.doi.org/10.1155/2016/8626987
dc.identifier.citationScientific World Journal, v. 2016.
dc.identifier.doi10.1155/2016/8626987
dc.identifier.file2-s2.0-84988974998.pdf
dc.identifier.issn1537-744X
dc.identifier.issn2356-6140
dc.identifier.scopus2-s2.0-84988974998
dc.identifier.urihttp://hdl.handle.net/11449/173539
dc.language.isoeng
dc.relation.ispartofScientific World Journal
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titlePunica granatum L. (Pomegranate) extract: In vivo study of antimicrobial activity against Porphyromonas gingivalis in galleria mellonella modelen
dc.typeArtigo
unesp.author.lattes0053567153623569[7]
unesp.author.orcid0000-0001-9478-8123[3]
unesp.author.orcid0000-0002-1747-6158[7]

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Artigos - Microbiologia e Imunologia - IBB