Immunohistochemical and molecular expression of laminin-332 gamma-2 chain in canine mammary tumors

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dc.contributor.authorZuccari, D. A. P. C.
dc.contributor.authorCastro, R.
dc.contributor.authorJardim, B. V. [UNESP]
dc.contributor.authorMancini, U. M.
dc.contributor.authorPolachini, G. M.
dc.contributor.institutionFaculdade de Medicina de São José do Rio Preto (FAMERP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:30:54Z
dc.date.available2014-05-20T15:30:54Z
dc.date.issued2011-02-01
dc.description.abstractForty-eight cases of canine mammary cancer were investigated to evaluate the immunohistochemical distribution of the gamma 2 chain of laminin-332. Tumor cells were compared to a pool of normal mammary tissues using quantitative RT-PCR. The western blot was performed in eight tumor samples as complementary test to evaluate protein integrity. Immunohistochemistry experiments showed negative, focal, and weak expression of laminin-332 gamma 2 in tumors with the worst prognosis. Quantitative PCR revealed downregulation of the gene in 27 (56.2%) of the animals. Out of the 16 dogs with gamma 2 chain overexpression, seven were still alive. The western blot results showed bands generation of 36, 50, and 98kDa, suggesting degradation of laminin-332 gamma 2 in malignant tumors. The results suggest that, in the future, low expression and/or degradation of laminin-332 gamma 2 chain in canine mammary tumors may be used as an indicator of malignant potential. However, further studies are necessary to corroborate these results.en
dc.description.affiliationFaculdade de Medicina de São José do Rio Preto (FAMERP), BR-5090000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUNESP, IBILCE, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, IBILCE, Sao Jose do Rio Preto, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 05/51936-0
dc.format.extent28-35
dc.identifierhttp://dx.doi.org/10.1590/S0102-09352011000100005
dc.identifier.citationArquivo Brasileiro de Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 63, n. 1, p. 28-35, 2011.
dc.identifier.fileS0102-09352011000100005.pdf
dc.identifier.issn0102-0935
dc.identifier.scieloS0102-09352011000100005
dc.identifier.urihttp://hdl.handle.net/11449/40187
dc.identifier.wosWOS:000289643900005
dc.language.isoeng
dc.publisherArquivo Brasileiro Medicina Veterinaria Zootecnia
dc.relation.ispartofArquivo Brasileiro de Medicina Veterinária e Zootecnia
dc.relation.ispartofjcr0.286
dc.relation.ispartofsjr0,248
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectdogen
dc.subjectadhesion moleculeen
dc.subjectcanceren
dc.subjectwestern bloten
dc.subjectRT-PCRen
dc.titleImmunohistochemical and molecular expression of laminin-332 gamma-2 chain in canine mammary tumorsen
dc.title.alternativeExpressão imunoistoquímica e molecular da laminina-332 cadeia gama-2 em tumores mamários de cadelaspt
dc.typeArtigo
dcterms.rightsHolderArquivo Brasileiro Medicina Veterinaria Zootecnia

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