Impact of Bifidobacterium animalis subsp. lactis HN019 probiotic in the prevention of periodontitis associated with immunosuppression

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dc.contributor.authorMaia, Luciana Prado
dc.contributor.authorde Almeida Silva Levi, Yara Loyanne
dc.contributor.authorHenrique Félix Silva, Pedro
dc.contributor.authorCarla Wons, Luana
dc.contributor.authorPizzo Pitelli, Livia
dc.contributor.authorGoulart de Castro, Jéssica
dc.contributor.authorda Silva Dólens, Eder
dc.contributor.authorGregorio, Danielle
dc.contributor.authorGouveia Straioto, Fabiana
dc.contributor.authordos Santos Santinoni, Carolina
dc.contributor.authorCasarin, Renato
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorAparecida Chaves Furlaneto, Flávia
dc.contributor.authorReis Messora, Michel
dc.contributor.institutionUNOESTE – University of Western São Paulo
dc.contributor.institutionUNOPAR – University Pitágoras UNOPAR
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T15:42:23Z
dc.date.available2023-07-29T15:42:23Z
dc.date.issued2023-03-01
dc.description.abstractBackground: This study evaluated the effects of the probiotic Bifidobacterium animalis subsp. lactis HN019 (HN019) in the development of experimental periodontitis (EP) in rats submitted to chemotherapy (5-fluorouracil [5FU]). Methods: Eighty male rats were divided into the following groups: control (C); treated with 5FU (60 mg/kg at day 30 and 40 mg/kg at day 32); treated with probiotic (HN019) (daily, for 44 days, starting at day 0); treatment with 5FU and probiotic (5FU-HN019); only EP (EP) (ligature placed on lower first molars at day 30, maintained for 14 days); EP and treatment with 5FU (EP-5FU); EP and treatment with probiotic (EP-HN019); and EP and treatment with 5FU and probiotic (EP-5FU-HN019). Euthanasia occurred at day 44. Morphometric, histomorphometric, microtomographic, immunohistochemical, immunoenzymatic, and gene expressions analyses were performed. The data obtained were statistically analyzed (p < 0.05). Results: The EP-5FU-HN019 group showed less bone and connective tissue loss when compared with EP-5FU group, while EP-HN019 and EP-5FU-HN019 groups had greater bone volume than EP and EP-5FU groups, respectively (p < 0.05). A decrease in immunostaining for tartrate-resistant acid phosphatase and RANKL, an increase for osteoprotegerin and lower interleukin-1β levels were observed in EP-5FU-HN019 group, when compared with EP-5FU group (p < 0.0001). Probiotic therapy led to an increase in the proportions of B. lactis in the feces (p = 0.0018), but not in the biofilm, and reduced the expression of Fusobacterium nucleatum and Prevotella intermedia in the biofilm (p < 0.0001). Conclusion: B. lactis HN019 reduced the severity of EP in rats submitted to chemotherapy, modulating immunoinflammatory parameters in periodontal tissues and reducing periodontopathogens expression on biofilm in rats submitted to chemotherapy.en
dc.description.affiliationGraduate Program in Dentistry UNOESTE – University of Western São Paulo, São Paulo
dc.description.affiliationGraduate Program in Dentistry UNOPAR – University Pitágoras UNOPAR, Paraná
dc.description.affiliationDepartment of Oral Surgery and Periodontology School of Dentistry of Ribeirão Preto University of São Paulo – USP, São Paulo
dc.description.affiliationDepartment of Prosthodontics and Periodontics School of Dentistry Campinas State University – UNICAMP, São Paulo
dc.description.affiliationDepartment of Basic Sciences School of Dentistry São Paulo State University - UNESP, São Paulo
dc.description.affiliationUnespDepartment of Basic Sciences School of Dentistry São Paulo State University - UNESP, São Paulo
dc.format.extent389-404
dc.identifierhttp://dx.doi.org/10.1002/JPER.22-0146
dc.identifier.citationJournal of Periodontology, v. 94, n. 3, p. 389-404, 2023.
dc.identifier.doi10.1002/JPER.22-0146
dc.identifier.issn0022-3492
dc.identifier.scopus2-s2.0-85144011035
dc.identifier.urihttp://hdl.handle.net/11449/249475
dc.language.isoeng
dc.relation.ispartofJournal of Periodontology
dc.sourceScopus
dc.subjectantineoplastic agents
dc.subjectbifidobacterium animalis
dc.subjectperiodontitis
dc.subjectprobiotics
dc.titleImpact of Bifidobacterium animalis subsp. lactis HN019 probiotic in the prevention of periodontitis associated with immunosuppressionen
dc.typeArtigo
unesp.author.orcid0000-0001-5697-2587[1]
unesp.author.orcid0000-0003-1566-5673[2]
unesp.author.orcid0000-0002-7583-7046[3]
unesp.author.orcid0000-0001-5153-2419[10]
unesp.author.orcid0000-0003-1743-5855[11]

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