Impact of Bifidobacterium animalis subsp. lactis HN019 probiotic in the prevention of periodontitis associated with immunosuppression
dc.contributor.author | Maia, Luciana Prado | |
dc.contributor.author | de Almeida Silva Levi, Yara Loyanne | |
dc.contributor.author | Henrique Félix Silva, Pedro | |
dc.contributor.author | Carla Wons, Luana | |
dc.contributor.author | Pizzo Pitelli, Livia | |
dc.contributor.author | Goulart de Castro, Jéssica | |
dc.contributor.author | da Silva Dólens, Eder | |
dc.contributor.author | Gregorio, Danielle | |
dc.contributor.author | Gouveia Straioto, Fabiana | |
dc.contributor.author | dos Santos Santinoni, Carolina | |
dc.contributor.author | Casarin, Renato | |
dc.contributor.author | Ervolino, Edilson [UNESP] | |
dc.contributor.author | Aparecida Chaves Furlaneto, Flávia | |
dc.contributor.author | Reis Messora, Michel | |
dc.contributor.institution | UNOESTE – University of Western São Paulo | |
dc.contributor.institution | UNOPAR – University Pitágoras UNOPAR | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.contributor.institution | Universidade Estadual de Campinas (UNICAMP) | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.date.accessioned | 2023-07-29T15:42:23Z | |
dc.date.available | 2023-07-29T15:42:23Z | |
dc.date.issued | 2023-03-01 | |
dc.description.abstract | Background: This study evaluated the effects of the probiotic Bifidobacterium animalis subsp. lactis HN019 (HN019) in the development of experimental periodontitis (EP) in rats submitted to chemotherapy (5-fluorouracil [5FU]). Methods: Eighty male rats were divided into the following groups: control (C); treated with 5FU (60 mg/kg at day 30 and 40 mg/kg at day 32); treated with probiotic (HN019) (daily, for 44 days, starting at day 0); treatment with 5FU and probiotic (5FU-HN019); only EP (EP) (ligature placed on lower first molars at day 30, maintained for 14 days); EP and treatment with 5FU (EP-5FU); EP and treatment with probiotic (EP-HN019); and EP and treatment with 5FU and probiotic (EP-5FU-HN019). Euthanasia occurred at day 44. Morphometric, histomorphometric, microtomographic, immunohistochemical, immunoenzymatic, and gene expressions analyses were performed. The data obtained were statistically analyzed (p < 0.05). Results: The EP-5FU-HN019 group showed less bone and connective tissue loss when compared with EP-5FU group, while EP-HN019 and EP-5FU-HN019 groups had greater bone volume than EP and EP-5FU groups, respectively (p < 0.05). A decrease in immunostaining for tartrate-resistant acid phosphatase and RANKL, an increase for osteoprotegerin and lower interleukin-1β levels were observed in EP-5FU-HN019 group, when compared with EP-5FU group (p < 0.0001). Probiotic therapy led to an increase in the proportions of B. lactis in the feces (p = 0.0018), but not in the biofilm, and reduced the expression of Fusobacterium nucleatum and Prevotella intermedia in the biofilm (p < 0.0001). Conclusion: B. lactis HN019 reduced the severity of EP in rats submitted to chemotherapy, modulating immunoinflammatory parameters in periodontal tissues and reducing periodontopathogens expression on biofilm in rats submitted to chemotherapy. | en |
dc.description.affiliation | Graduate Program in Dentistry UNOESTE – University of Western São Paulo, São Paulo | |
dc.description.affiliation | Graduate Program in Dentistry UNOPAR – University Pitágoras UNOPAR, Paraná | |
dc.description.affiliation | Department of Oral Surgery and Periodontology School of Dentistry of Ribeirão Preto University of São Paulo – USP, São Paulo | |
dc.description.affiliation | Department of Prosthodontics and Periodontics School of Dentistry Campinas State University – UNICAMP, São Paulo | |
dc.description.affiliation | Department of Basic Sciences School of Dentistry São Paulo State University - UNESP, São Paulo | |
dc.description.affiliationUnesp | Department of Basic Sciences School of Dentistry São Paulo State University - UNESP, São Paulo | |
dc.format.extent | 389-404 | |
dc.identifier | http://dx.doi.org/10.1002/JPER.22-0146 | |
dc.identifier.citation | Journal of Periodontology, v. 94, n. 3, p. 389-404, 2023. | |
dc.identifier.doi | 10.1002/JPER.22-0146 | |
dc.identifier.issn | 0022-3492 | |
dc.identifier.scopus | 2-s2.0-85144011035 | |
dc.identifier.uri | http://hdl.handle.net/11449/249475 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Periodontology | |
dc.source | Scopus | |
dc.subject | antineoplastic agents | |
dc.subject | bifidobacterium animalis | |
dc.subject | periodontitis | |
dc.subject | probiotics | |
dc.title | Impact of Bifidobacterium animalis subsp. lactis HN019 probiotic in the prevention of periodontitis associated with immunosuppression | en |
dc.type | Artigo | |
unesp.author.orcid | 0000-0001-5697-2587[1] | |
unesp.author.orcid | 0000-0003-1566-5673[2] | |
unesp.author.orcid | 0000-0002-7583-7046[3] | |
unesp.author.orcid | 0000-0001-5153-2419[10] | |
unesp.author.orcid | 0000-0003-1743-5855[11] |