The Mechanism of Oocyte Activation Influences the Cell Cycle-Related Genes Expression During Bovine Preimplantation Development

Página do item simplificado
dc.contributor.authorMilazzotto, Marcella Pecora
dc.contributor.authorFeitosa, Weber Beringui
dc.contributor.authorPaula-Lopes, Fabiola Feitosa
dc.contributor.authorBuratini, Jose [UNESP]
dc.contributor.authorVisintin, Jose Antonio
dc.contributor.authorAssumpcao, Mayra E. O. A.
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:50:01Z
dc.date.available2014-05-20T13:50:01Z
dc.date.issued2012-10-01
dc.description.abstractThe first cleavage divisions and preimplantation embryonic development are supported by mRNA and proteins synthesized and stored during oogenesis. Thus, mRNA molecules of maternal origin decrease and embryonic development becomes gradually dependent on expression of genetic information derived from the embryonic genome. However, it is still unclear what the role of the sperm cell is during this phase and whether the absence of the sperm cell during the artificial oocyte activation affects subsequent embryonic development. The objective of this study was to determine, in bovine embryos, changes in cell cycle-associated transcript levels (cyclin A, cyclin B, cyclin E, CDC2, CDK2, and CDK4) after oocyte activation in the presence or absence of the sperm cell. To evaluate that, in vitro-produced (IVP) and parthenogenetically activated (PA) embryos (2-4 cells (2-4C), 8-16 cells (8-16C) and blastocysts) were evaluated by real-time PCR. There was no difference in cleavage and blastocyst rates between IVP and PA groups. Transcript level was higher in oocytes than in IVP and PA embryos. Cleaved PA embryos showed higher expression of cyclin A, cyclin B, cyclin E, and CDK2 and lower expression of CDC2 when compared with that from the IVP group. At the time of activation, all transcripts were expressed less in PA than in IVP embryos, whereas at the blastocyst stage, almost all genes were expressed at a higher level in the PA group. These results suggest that in both groups there is an initial consumption of these transcripts in the early stages of embryonic development. Furthermore, 8-16C embryos seem to synthesize more cell cycle-related genes than 2-4C embryos. However, in PA embryos, activation of the cell cycle genes seems to occur after the 8- to 16-cell stage, suggesting a failure in the activation process.en
dc.description.affiliationUniversidade Federal do ABC (UFABC), Nat & Human Sci Ctr, Santo Andre, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Anim Reprod, Fac Vet Med, São Paulo, Brazil
dc.description.affiliationUniv Fed São Paulo, Dept Biol Sci, Diadema, SP, Brazil
dc.description.affiliationUNESP, Dept Physiol, Inst Biosci, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Physiol, Inst Biosci, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 07/58456-9
dc.format.extent418-424
dc.identifierhttp://dx.doi.org/10.1089/cell.2012.0024
dc.identifier.citationCellular Reprogramming. New Rochelle: Mary Ann Liebert Inc., v. 14, n. 5, p. 418-424, 2012.
dc.identifier.doi10.1089/cell.2012.0024
dc.identifier.fileWOS000309545700005.pdf
dc.identifier.issn2152-4971
dc.identifier.urihttp://hdl.handle.net/11449/17835
dc.identifier.wosWOS:000309545700005
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc.
dc.relation.ispartofCellular Reprogramming
dc.relation.ispartofjcr1.430
dc.relation.ispartofsjr0,549
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleThe Mechanism of Oocyte Activation Influences the Cell Cycle-Related Genes Expression During Bovine Preimplantation Developmenten
dc.typeArtigo
dcterms.licensehttp://www.liebertpub.com/archpolicy/journal-of-womens-health/42/
dcterms.rightsHolderMary Ann Liebert Inc.
unesp.author.orcid0000-0002-7020-694X[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000309545700005.pdf
Tamanho:
308.06 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Fisiologia - IBB