Polymer induced condensation of DNA supercoils
dc.contributor.author | Bessa Ramos, Jose Esio [UNESP] | |
dc.contributor.author | Ruggiero Neto, Joao [UNESP] | |
dc.contributor.author | de Vries, Renko | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Wageningen Univ | |
dc.date.accessioned | 2014-05-20T14:02:41Z | |
dc.date.available | 2014-05-20T14:02:41Z | |
dc.date.issued | 2008-11-14 | |
dc.description.abstract | Macromolecular crowding is thought to be a significant factor driving DNA condensation in prokaryotic cells. Whereas DNA in prokaryotes is supercoiled, studies on crowding-induced DNA condensation have so far focused on linear DNA. Here we compare DNA condensation by poly(ethylene oxide) for supercoiled and linearized pUC18 plasmid DNA. It is found that supercoiling has only a limited influence on the critical amount of PEO needed to condense plasmid DNA. In order to pack DNA supercoils in condensates, it seems inevitable that they must be deformed in one way or another, to facilitate dense packing of DNA. Analytical estimates and Monte Carlo simulations indicate that packing of DNA supercoils in condensates is most likely facilitated by a decrease of the superhelical diameter rather than by unwinding of the supercoils. | en |
dc.description.affiliation | Univ Estadual Paulista, Dept Phys, IBILCE UNESP, BR-15054000 Sao Jose do Rio Preto, SP, Brazil | |
dc.description.affiliation | Wageningen Univ, Lab Phys Chem & Colloid Sci, NL-6700 EK Wageningen, Netherlands | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Dept Phys, IBILCE UNESP, BR-15054000 Sao Jose do Rio Preto, SP, Brazil | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.format.extent | 6 | |
dc.identifier | http://dx.doi.org/10.1063/1.2998521 | |
dc.identifier.citation | Journal of Chemical Physics. Melville: Amer Inst Physics, v. 129, n. 18, p. 6, 2008. | |
dc.identifier.doi | 10.1063/1.2998521 | |
dc.identifier.file | WOS000260944300053.pdf | |
dc.identifier.issn | 0021-9606 | |
dc.identifier.uri | http://hdl.handle.net/11449/22097 | |
dc.identifier.wos | WOS:000260944300053 | |
dc.language.iso | eng | |
dc.publisher | American Institute of Physics (AIP) | |
dc.relation.ispartof | Journal of Chemical Physics | |
dc.relation.ispartofjcr | 2.843 | |
dc.relation.ispartofsjr | 1,252 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | aggregation | en |
dc.subject | biochemistry | en |
dc.subject | cellular biophysics | en |
dc.subject | DNA | en |
dc.subject | Molecular biophysics | en |
dc.subject | molecular configurations | en |
dc.subject | Monte Carlo methods | en |
dc.subject | Polymers | en |
dc.title | Polymer induced condensation of DNA supercoils | en |
dc.type | Artigo | |
dcterms.license | http://www.aip.org/pubservs/web_posting_guidelines.html | |
dcterms.rightsHolder | Amer Inst Physics | |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Preto | pt |
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