Polymer induced condensation of DNA supercoils

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dc.contributor.authorBessa Ramos, Jose Esio [UNESP]
dc.contributor.authorRuggiero Neto, Joao [UNESP]
dc.contributor.authorde Vries, Renko
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionWageningen Univ
dc.date.accessioned2014-05-20T14:02:41Z
dc.date.available2014-05-20T14:02:41Z
dc.date.issued2008-11-14
dc.description.abstractMacromolecular crowding is thought to be a significant factor driving DNA condensation in prokaryotic cells. Whereas DNA in prokaryotes is supercoiled, studies on crowding-induced DNA condensation have so far focused on linear DNA. Here we compare DNA condensation by poly(ethylene oxide) for supercoiled and linearized pUC18 plasmid DNA. It is found that supercoiling has only a limited influence on the critical amount of PEO needed to condense plasmid DNA. In order to pack DNA supercoils in condensates, it seems inevitable that they must be deformed in one way or another, to facilitate dense packing of DNA. Analytical estimates and Monte Carlo simulations indicate that packing of DNA supercoils in condensates is most likely facilitated by a decrease of the superhelical diameter rather than by unwinding of the supercoils.en
dc.description.affiliationUniv Estadual Paulista, Dept Phys, IBILCE UNESP, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationWageningen Univ, Lab Phys Chem & Colloid Sci, NL-6700 EK Wageningen, Netherlands
dc.description.affiliationUnespUniv Estadual Paulista, Dept Phys, IBILCE UNESP, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent6
dc.identifierhttp://dx.doi.org/10.1063/1.2998521
dc.identifier.citationJournal of Chemical Physics. Melville: Amer Inst Physics, v. 129, n. 18, p. 6, 2008.
dc.identifier.doi10.1063/1.2998521
dc.identifier.fileWOS000260944300053.pdf
dc.identifier.issn0021-9606
dc.identifier.urihttp://hdl.handle.net/11449/22097
dc.identifier.wosWOS:000260944300053
dc.language.isoeng
dc.publisherAmerican Institute of Physics (AIP)
dc.relation.ispartofJournal of Chemical Physics
dc.relation.ispartofjcr2.843
dc.relation.ispartofsjr1,252
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectaggregationen
dc.subjectbiochemistryen
dc.subjectcellular biophysicsen
dc.subjectDNAen
dc.subjectMolecular biophysicsen
dc.subjectmolecular configurationsen
dc.subjectMonte Carlo methodsen
dc.subjectPolymersen
dc.titlePolymer induced condensation of DNA supercoilsen
dc.typeArtigo
dcterms.licensehttp://www.aip.org/pubservs/web_posting_guidelines.html
dcterms.rightsHolderAmer Inst Physics
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Física - IBILCE