Fine needle aspirate cell blocks are reliable for detection of hormone receptors and HER-2 by immunohistochemistry in breast carcinoma

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dc.contributor.authorBueno Angela, S. P. [UNESP]
dc.contributor.authorViero, Rosa Marlene [UNESP]
dc.contributor.authorSoares, C. T.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto Lauro de Souza Lima (ILSL)
dc.date.accessioned2014-05-27T11:28:19Z
dc.date.available2014-05-27T11:28:19Z
dc.date.issued2013-02-01
dc.description.abstractAims: To evaluate the reliability of fine needle aspirate cell blocks in the assessment of oestrogen receptor (ER), progesterone receptor (PR) and HER-2/neu proteins by immunohistochemistry in comparison with surgical specimens. Materials and methods: This is a retrospective study of 62 cases of breast carcinoma diagnosed by fine needle aspiration cytology (FNAC) and confirmed using the surgical specimen. Immunohistochemical tests were performed to assess the presence of oestrogen receptor (ER), progesterone receptor (PR) and HER-2/neu proteins in cell blocks and the corresponding surgical specimens. The cell block method used alcohol prior to formalin fixation. Cases with 10% or more stained cells were considered positive for ER and PR. Positivity for HER-2/neu was assessed on a scale of 0-3+. The criterion for positivity was a score of 3+. Results: Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy of the cell blocks in the investigation of ER, PR and HER-2/neu protein (3+) were (%): ER, 92.7, 85.7, 92.7, 85.7 and 90.3; PR, 92.7, 94.7, 97.4, 87.0 and 93.5; HER-2/neu, 70.0, 100.0, 100.0, 94.5 and 95.2. Discrepancies were seen in cell blocks in the 1+ and 2+ HER-2/neu staining scores: two of 12 cases scoring 2+ and one case of 26 scoring 1+ on cell blocks scored 3+ on surgical specimens. The correlation index between cell block and corresponding surgical specimen varied from 90% to 94%. Conclusion: Cell blocks provide a useful method of assessing ER, PR and HER-2/neu, mainly for inoperable and recurrent cases, but consideration should be given to carrying out FISH analysis on 1+ as well as 2+ HER-2/neu results. © 2012 Blackwell Publishing Ltd.en
dc.description.affiliationDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP), Botucatu, São Paulo
dc.description.affiliationDepartment of Anatomical Pathology 'Lauro de Souza Lima' Institute, Bauru, São Paulo
dc.description.affiliationUnespDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP), Botucatu, São Paulo
dc.format.extent26-32
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2303.2011.00934.x
dc.identifier.citationCytopathology, v. 24, n. 1, p. 26-32, 2013.
dc.identifier.doi10.1111/j.1365-2303.2011.00934.x
dc.identifier.issn0956-5507
dc.identifier.issn1365-2303
dc.identifier.lattes1453564171333848
dc.identifier.scopus2-s2.0-84872680523
dc.identifier.urihttp://hdl.handle.net/11449/74498
dc.identifier.wosWOS:000313873900005
dc.language.isoeng
dc.relation.ispartofCytopathology
dc.relation.ispartofjcr1.376
dc.relation.ispartofsjr0,509
dc.relation.ispartofsjr0,509
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectBreast carcinoma
dc.subjectCell block
dc.subjectFine needle aspiration cytology
dc.subjectHER-2/neu
dc.subjectHormone receptors
dc.subjectImmunocytochemistry
dc.subjectepidermal growth factor receptor 2
dc.subjectestrogen receptor
dc.subjectprogesterone receptor
dc.subjectaccuracy
dc.subjectbreast carcinoma
dc.subjectcancer cell
dc.subjectcancer tissue
dc.subjectcontrolled study
dc.subjectfine needle aspirate cell block
dc.subjectfine needle aspiration biopsy
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectmajor clinical study
dc.subjectpredictive value
dc.subjectpriority journal
dc.subjectreliability
dc.subjectretrospective study
dc.subjectsensitivity and specificity
dc.subjectBiopsy, Fine-Needle
dc.subjectBreast Neoplasms
dc.subjectFemale
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectNeoplasm Grading
dc.subjectReceptor, erbB-2
dc.subjectReceptors, Estrogen
dc.subjectReceptors, Progesterone
dc.subjectReproducibility of Results
dc.subjectRetrospective Studies
dc.subjectSensitivity and Specificity
dc.subjectStaining and Labeling
dc.subjectTumor Markers, Biological
dc.titleFine needle aspirate cell blocks are reliable for detection of hormone receptors and HER-2 by immunohistochemistry in breast carcinomaen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.author.lattes1453564171333848
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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