Root instrumentation with an erbium : yttrium-aluminum-garnet laser: Effect on the morphology of fibroblasts

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dc.contributor.authorRossa, C.
dc.contributor.authorSilverio, K. G.
dc.contributor.authorZanin, ICJ
dc.contributor.authorBrugnera, A.
dc.contributor.authorSampaio, JEC
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniv Camilo Castelo Branco
dc.date.accessioned2014-05-20T15:30:42Z
dc.date.available2014-05-20T15:30:42Z
dc.date.issued2002-07-01
dc.description.abstractObjective: the purpose of this study was to evaluate the effect of erbium:yttrium-aluminum-garnet laser instrumentation of root surfaces on the morphology of fibroblasts from continuous lineage. Method and materials: Dentinal slices with 4 mm(2) of surface area were obtained from teeth extracted for severe periodontal involvement. Specimens were assigned to one of three treatment groups: group 1, application of the laser with an energy level of 250 mJ at 103 pulses per second; group 2, application of the laser with an energy level of 80 mJ at 166 pulses per second; and group 3, similar to group 2, but with concomitant water irrigation of the device. The specimens were incubated in multiwell plates containing cell culture media. After 24 hours, the specimens were submitted to routine preparation for scanning electron microscopy. Three independent and blind examiners used photomicrographs to evaluate the morphology of the fibroblasts: 0 = without cells; 1 = flat cells; 2 = round cells; and 3 = combination of round and flat cells. Results: Statistical analysis indicated that there were significant differences among treatment groups and that group 3 was significantly different from groups 1 and 2. Conclusion: There was no difference between groups 1 and 2 in the morphology of fibroblasts. Laser instrumentation with concomitant irrigation impaired the adhesion of fibroblasts to dentinal surfaces.en
dc.description.affiliationState Univ São Paulo, Sch Dent Araraquara, Dept Diag & Surg, BR-14801903 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Sch Dent Piracicaba, São Paulo, Brazil
dc.description.affiliationUniv Camilo Castelo Branco, Sch Dent, Dept Laser, São Paulo, Brazil
dc.description.affiliationUnespState Univ São Paulo, Sch Dent Araraquara, Dept Diag & Surg, BR-14801903 São Paulo, Brazil
dc.format.extent496-502
dc.identifierhttp://www.quintpub.com/journals/qi/abstract.php?iss2_id=404&article_id=5020&article=3&title=Root#.UulapbRTvIU
dc.identifier.citationQuintessence International. Hanover Park: Quintessence Publishing Co Inc., v. 33, n. 7, p. 496-502, 2002.
dc.identifier.issn0033-6572
dc.identifier.lattes9531839078426485
dc.identifier.urihttp://hdl.handle.net/11449/40018
dc.identifier.wosWOS:000177091500003
dc.language.isoeng
dc.publisherQuintessence Publishing Co Inc
dc.relation.ispartofQuintessence International
dc.relation.ispartofjcr1.088
dc.relation.ispartofsjr0,563
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectfibroblastpt
dc.subjectlaserpt
dc.subjectperiodontal diseasept
dc.subjectperiodontal therapypt
dc.subjecttooth rootpt
dc.titleRoot instrumentation with an erbium : yttrium-aluminum-garnet laser: Effect on the morphology of fibroblastsen
dc.typeArtigo
dcterms.licensehttp://www.quintpub.com/terms.php
dcterms.rightsHolderQuintessence Publishing Co Inc
unesp.author.lattes9531839078426485
unesp.author.lattes7634063102292261[1]
unesp.author.orcid0000-0003-1705-5481[1]
unesp.author.orcid0000-0001-5271-9808[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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