Influence of age and gender on alveolar bone healing post tooth extraction in 129 Sv mice: a microtomographic, histological, and biochemical characterization

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dc.contributor.authorBiguetti, Claudia Cristina [UNESP]
dc.contributor.authorLakkasetter Chandrashekar, Bhuvana
dc.contributor.authorSimionato, Gustavo Baroni [UNESP]
dc.contributor.authorMomesso, Nataira Regina [UNESP]
dc.contributor.authorDuarte, Marco Antonio Hungaro
dc.contributor.authorRodrigues, Danieli C.
dc.contributor.authorMatsumoto, Mariza Akemi [UNESP]
dc.contributor.institutionUniversity of Texas Rio Grande Valley
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of at Dallas
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2023-07-29T13:57:40Z
dc.date.available2023-07-29T13:57:40Z
dc.date.issued2023-01-01
dc.description.abstractObjectives: To analyze the effect of biological sex and aging on craniofacial bone features in 129 Sv mice and their influence on dental socket healing post tooth extraction. Materials and methods: A total of 52 129 Sv mice were used, of which 28 were young (3–4 months) and 24 were aged (17–18 months), equally distributed according to biological sex. After an upper right incisor extraction, mice specimens were collected at 7, 14, and 21-days post-surgery for microtomographic (microCT) and comprehensive histological analysis. Mandible, skull bones, and maxillae at 21 days were analyzed by microCT, while blood plasma samples were collected for the detection of key bone turnover markers (P1NP and CTX-1) by enzyme-linked immunosorbent (ELISA) assay. Results: Aged females depicted significantly decreased mineralized bone content in alveolar sockets in comparison to young females and aged males at day 7, and aged males at day 14. Mandible RCA and Ma.AR of aged females were also significantly decreased in comparison with young females. Histological evaluation revealed that all alveolar sockets healed at 21 days with inflammation resolution and deposition of new bone. Immunohistochemistry for TRAP revealed increased area density for osteoclasts in alveolar sockets of aged females when compared to young females at 21 days. While a significant increase in CTX-1 levels was detected in blood plasma of aged females when compared to young females, P1NP levels did not significantly change between young and older females. No significant changes were observed for males. Conclusions: Age and gender can significantly affect craniofacial bones of 129 Sv mice, especially maxilla and mandible in females. Considering the altered bone resorption parameters and delayed alveolar bone healing in older females, careful deliberation is necessary during development of pre-clinical models for craniofacial research. Clinical relevance: Aging can be a contributing factor to slower bone healing in craniofacial bones. However, there are no sufficient experimental studies that have addressed this phenomenon along with biological sex taken into consideration.en
dc.description.affiliationDepartment of Surgery and Biomechanics School of Podiatric Medicine University of Texas Rio Grande Valley
dc.description.affiliationDepartment of Basic Sciences School of Dentistry of Araçatuba São Paulo State University (UNESP), São Paulo
dc.description.affiliationDepartment of Bioengineering University of at Dallas
dc.description.affiliationDepartment of Dental Materials and Endodontics School of Dentistry of Bauru University of Sao Paulo (USP), São Paulo
dc.description.affiliationUnespDepartment of Basic Sciences School of Dentistry of Araçatuba São Paulo State University (UNESP), São Paulo
dc.identifierhttp://dx.doi.org/10.1007/s00784-023-05087-y
dc.identifier.citationClinical Oral Investigations.
dc.identifier.doi10.1007/s00784-023-05087-y
dc.identifier.issn1436-3771
dc.identifier.issn1432-6981
dc.identifier.scopus2-s2.0-85160821645
dc.identifier.urihttp://hdl.handle.net/11449/248929
dc.language.isoeng
dc.relation.ispartofClinical Oral Investigations
dc.sourceScopus
dc.subjectAging
dc.subjectBone tissue
dc.subjectMice
dc.subjectSexual dimorphism
dc.subjectWound healing
dc.titleInfluence of age and gender on alveolar bone healing post tooth extraction in 129 Sv mice: a microtomographic, histological, and biochemical characterizationen
dc.typeArtigo

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