Histochemical and molecular evaluation of the prevalence of Leishmania spp. in hematophagous insects

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dc.contributor.authorCoelho, Willian Marinho Dourado
dc.contributor.authorBuzetti, Wilma Aparecida Starke [UNESP]
dc.contributor.authorBresciani, Katia Denise Saraiva [UNESP]
dc.contributor.institutionFCAA
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:06:16Z
dc.date.available2018-12-11T17:06:16Z
dc.date.issued2016-06-01
dc.description.abstractThe prevalence study of Leishmania spp. in hematophagous insects captured from the environment in bat roosts and pigeon nests, or feeding their hosts (cattle, pigs, horses, dogs and humans) in urban, peri-urban and rural areas, between 2012 and 2014. For this study, the amastigotes present in these insects were detected by histochemical and PCR techniques. Positive gene amplification for Leishmania was found in two horseflies of the species Tabanus importunus collected in the environment, and amastigote forms of Leishmania spp., as well as erythrocytes and leukocytes, were histochemically detected in one of that insect. The other analyzed insects were not positive by PCR our by direct parasitological examination. Only horseflies captured in urban and peri-urban areas were positive. During the collection, no phlebotomine sand flies were captured in rural areas far from the city limits. It can be concluded that the discovery of horseflies positive for Leishmania spp. in urban and peri-urban areas indicates the likelihood that urban areas and their surroundings provide vector parasites with an environment suitable for the spread and consequent perpetuation of the biological cycle of this protozoan.en
dc.description.affiliationFaculdade de Ciências Agrárias de Andradina FCAA, Rua Amazonas, 501
dc.description.affiliationDepartamento de Biologia e Zootecnia FEIS-UNESP-Campus de Ilha Solteira
dc.description.affiliationDepartamento de Apoio Produção e Saúde Animal FOA UNESP, Rua Clóvis Pestana, 793
dc.description.affiliationUnespDepartamento de Biologia e Zootecnia FEIS-UNESP-Campus de Ilha Solteira
dc.description.affiliationUnespDepartamento de Apoio Produção e Saúde Animal FOA UNESP, Rua Clóvis Pestana, 793
dc.format.extent85-89
dc.identifierhttp://dx.doi.org/10.1016/j.parepi.2016.04.004
dc.identifier.citationParasite Epidemiology and Control, v. 1, n. 2, p. 85-89, 2016.
dc.identifier.doi10.1016/j.parepi.2016.04.004
dc.identifier.file2-s2.0-84989914948.pdf
dc.identifier.issn2405-6731
dc.identifier.lattes5950594366829647
dc.identifier.scopus2-s2.0-84989914948
dc.identifier.urihttp://hdl.handle.net/11449/173556
dc.language.isoeng
dc.relation.ispartofParasite Epidemiology and Control
dc.relation.ispartofsjr0,525
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCulex spp.
dc.subjectLeishmaniasis
dc.subjectSimulium spp.
dc.subjectTriatoma sordida
dc.subjectVectors
dc.titleHistochemical and molecular evaluation of the prevalence of Leishmania spp. in hematophagous insectsen
dc.typeArtigo
unesp.author.lattes5950594366829647

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Artigos - Biologia e Zootecnia - FEIS