Migratory route of Strongyloides venezuelensis in Lewis rats: Comparison of histological analyses and PCR

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dc.contributor.authorMarra, Nelson Mendes [UNESP]
dc.contributor.authorChiuso-Minicucci, Fernanda [UNESP]
dc.contributor.authorMachado, Gabriel Capella [UNESP]
dc.contributor.authorGoncalves Zorzella-Pezavento, Sofia Fernanda [UNESP]
dc.contributor.authorDonega Franca, Thais Graziela [UNESP]
dc.contributor.authorWatanabe Ishikawa, Larissa Lumi [UNESP]
dc.contributor.authorAmarante, Alessandro Francisco Talamini do [UNESP]
dc.contributor.authorSartori, Alexandrina [UNESP]
dc.contributor.authorAmarante, Monica R. V. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:51:05Z
dc.date.available2014-05-20T13:51:05Z
dc.date.issued2011-02-01
dc.description.abstractStrongyloides venezuelensis is a parasitic nematode that has been used as a model to study human and animal strongyloidiasis. In this study, we compared the sensitivity between traditional methodologies and PCR assay to characterize the dynamics of S. venezuelensis infection and its migration route in Lewis rats subcutaneously infected with 4000 L3. The dynamics of the infection was determined by counting the number of eggs and by detecting parasite deoxyribonucleic acid in faeces samples. Both techniques similarly detected the infection at day 6 after larvae inoculation. However. PCR performed with the genus primer showed higher sensitivity during the recovery phase. Histological analysis and PCR assay were then used to follow parasite tissue migration. S. venezuelensis migration route included the muscular fibers below the skin, the pulmonary alveoli and the small intestine vilosities. The sensitivity of these two techniques to detect parasite's presence in these tissues was statistically similar. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista UNESP, Dept Microbiol & Imunol, Inst Biociencias, BR-18618000 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Dept Parasitol, Biosci Inst, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Dept Microbiol & Imunol, Inst Biociencias, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Dept Parasitol, Biosci Inst, BR-18618000 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.format.extent334-339
dc.identifierhttp://dx.doi.org/10.1016/j.exppara.2010.08.006
dc.identifier.citationExperimental Parasitology. San Diego: Academic Press Inc. Elsevier B.V., v. 127, n. 2, p. 334-339, 2011.
dc.identifier.doi10.1016/j.exppara.2010.08.006
dc.identifier.issn0014-4894
dc.identifier.lattes2677231663329706
dc.identifier.lattes4977572416129527
dc.identifier.orcid0000-0002-5929-1223
dc.identifier.urihttp://hdl.handle.net/11449/18240
dc.identifier.wosWOS:000286643300002
dc.language.isoeng
dc.publisherAcademic Press Inc. Elsevier B.V.
dc.relation.ispartofExperimental Parasitology
dc.relation.ispartofjcr1.821
dc.relation.ispartofsjr0,635
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectStrongyloides venezuelensisen
dc.subjectMigratory routeen
dc.subjectLewis ratsen
dc.subjectHistological analysisen
dc.subjectPCRen
dc.titleMigratory route of Strongyloides venezuelensis in Lewis rats: Comparison of histological analyses and PCRen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderAcademic Press Inc. Elsevier B.V.
unesp.author.lattes2677231663329706[7]
unesp.author.lattes4977572416129527[8]
unesp.author.orcid0000-0002-5929-1223[9]
unesp.author.orcid0000-0003-3995-5501[7]
unesp.author.orcid0000-0003-4557-3331[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Parasitologia - IBB
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