Application of natural rubber latex as scaffold for osteoblast to guided bone regeneration

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dc.contributor.authorBorges, Felipe Azevedo [UNESP]
dc.contributor.authorde Barros, Natan Roberto [UNESP]
dc.contributor.authorGarms, Bruna Cambraia [UNESP]
dc.contributor.authorMiranda, Matheus Carlos Romeiro [UNESP]
dc.contributor.authorGemeinder, Jose Lucio Padua [UNESP]
dc.contributor.authorRibeiro-Paes, João Tadeu [UNESP]
dc.contributor.authorSilva, Rodrigo Ferreira
dc.contributor.authorde Toledo, Karina Alves [UNESP]
dc.contributor.authorHerculano, Rondinelli Donizetti [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-12-11T17:12:38Z
dc.date.available2018-12-11T17:12:38Z
dc.date.issued2017-10-15
dc.description.abstractNatural rubber latex (NRL) from Hevea brasiliensis is a colloidal system composed of cis-1,4-polyisoprene. Its applications have grown due its angiogenic and wound healing activity. NRL has been used in guided bone regeneration as barrier, enhancing bone formation. However, there has been no study reported so far which shows its in vitro biocompatibility with osteoblasts. Thus, the aim of this work was to apply thermally induced phase separation under several temperatures to induce porosity in NRL; and test its mechanical properties, cytotoxicity, cell adhesion, and mineralization with MC3T3-E1. Only biomembranes submitted at −20 and −10 °C presented porosity. Fourier transform infrared spectroscopy showed no change in cis-1,4-isoprene spectra. Biomembranes were elastic (Young's modulus < 1 MPa). According to ISO10993–5, NRL showed no cytotoxicity. Cells adhered on the NRL and produced mineral matrix as analyzed by scanning electron microscopy–energy-dispersive spectrometry, von kossa, and Fourier transform infrared spectroscopy. Cells on NRL presented higher alkaline phosphatase activity, however, mineralization showed no difference by alizarin red S dye extraction. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45321.en
dc.description.affiliationInstituto de Química Faculdade de Ciências e Letras UNESP Univ Estadual Paulista Campus Araraquara, Rua Prof. Francisco Degni no. 55
dc.description.affiliationDepartamento de Ciências Biológicas Faculdade de Ciências e Letras de Assis UNESP Univ Estadual Paulista, Campus Assis. Av. Dom Antonio no. 2100
dc.description.affiliationDepartamento de Química Faculdade de Filosofia Ciêcias e Letras de Ribeirão Preto USP Univ de São Paulo, Av. Bandeirantes no. 3900
dc.description.affiliationDepartamento de Bioprocessos e Biotecnologia Faculdade de Ciências Farmacêuticas de Araraquara UNESP Univ Estadual Paulista Campus Araraquara, Rodovia Araraquara Jaú, Km 01-s/n
dc.description.affiliationUnespInstituto de Química Faculdade de Ciências e Letras UNESP Univ Estadual Paulista Campus Araraquara, Rua Prof. Francisco Degni no. 55
dc.description.affiliationUnespDepartamento de Ciências Biológicas Faculdade de Ciências e Letras de Assis UNESP Univ Estadual Paulista, Campus Assis. Av. Dom Antonio no. 2100
dc.description.affiliationUnespDepartamento de Bioprocessos e Biotecnologia Faculdade de Ciências Farmacêuticas de Araraquara UNESP Univ Estadual Paulista Campus Araraquara, Rodovia Araraquara Jaú, Km 01-s/n
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 11/17411–8
dc.description.sponsorshipIdFAPESP: 14/17526–8
dc.identifierhttp://dx.doi.org/10.1002/app.45321
dc.identifier.citationJournal of Applied Polymer Science, v. 134, n. 39, 2017.
dc.identifier.doi10.1002/app.45321
dc.identifier.issn1097-4628
dc.identifier.issn0021-8995
dc.identifier.scopus2-s2.0-85020422098
dc.identifier.urihttp://hdl.handle.net/11449/174736
dc.language.isoeng
dc.relation.ispartofJournal of Applied Polymer Science
dc.relation.ispartofsjr0,543
dc.relation.ispartofsjr0,543
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectbiocompatibility
dc.subjectbioengineering
dc.subjectmembranes
dc.subjectporous materials
dc.subjectrubber
dc.titleApplication of natural rubber latex as scaffold for osteoblast to guided bone regenerationen
dc.typeArtigo
unesp.author.orcid0000-0002-6575-3041[1]

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Artigos - Ciências Biológicas - FCLAS
Artigos - Bioquímica e Tecnologia - IQ