Candida/Candida biofilms. First description of dual-species Candida albicans/C. rugosa biofilm

Página do item simplificado
dc.contributor.authorMartins, Carlos Henrique Gomes
dc.contributor.authorPires, Regina Helena [UNESP]
dc.contributor.authorCunha, Aline Oliveira
dc.contributor.authorPereira, Cristiane Aparecida Martins
dc.contributor.authorSingulani, Junya de Lacorte [UNESP]
dc.contributor.authorAbrão, Fariza
dc.contributor.authorMoraes, Thais de
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.institutionUNIRAN
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:27:14Z
dc.date.available2018-12-11T17:27:14Z
dc.date.issued2016-04-01
dc.description.abstractDenture liners have physical properties that favour plaque accumulation and colonization by Candida species, irritating oral tissues and causing denture stomatitis. To isolate and determine the incidence of oral Candida species in dental prostheses, oral swabs were collected from the dental prostheses of 66 patients. All the strains were screened for their ability to form biofilms; both monospecies and dual-species combinations were tested. Candida albicans (63 %) was the most frequently isolated microorganism; Candida tropicalis (14 %), Candida glabrata (13 %), Candida rugosa (5 %), Candida parapsilosis (3 %), and Candida krusei (2 %) were also detected. The XTT assay showed that C. albicans SC5314 possessed a biofilm-forming ability significantly higher (p < 0.001) than non-albicans Candida strains, after 6 h 37 °C. The total C. albicans CFU from a dual-species biofilm was less than the total CFU of a monospecies C. albicans biofilm. In contrast to the profuse hyphae verified in monospecies C. albicans biofilms, micrographies showed that the C. albicans/non-albicans Candida biofilms consisted of sparse yeast forms and profuse budding yeast cells that generated a network. These results suggested that C. albicans and the tested Candida species could co-exist in biofilms displaying apparent antagonism. The study provide the first description of C. albicans/C. rugosa mixed biofilm.en
dc.description.affiliationLaboratorio de Pesquisa em Microbiologia Aplicada Universidade de Franca UNIRAN, Av. Dr. Armando Salles de Oliveira, 201, Pq. Universitario
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Farmacêuticas UNESP, Rodovia Ararquara-Jaú, km 1
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Farmacêuticas UNESP, Rodovia Ararquara-Jaú, km 1
dc.format.extent530-537
dc.identifierhttp://dx.doi.org/10.1016/j.funbio.2016.01.013
dc.identifier.citationFungal Biology, v. 120, n. 4, p. 530-537, 2016.
dc.identifier.doi10.1016/j.funbio.2016.01.013
dc.identifier.file2-s2.0-84958581117.pdf
dc.identifier.issn1878-6146
dc.identifier.scopus2-s2.0-84958581117
dc.identifier.urihttp://hdl.handle.net/11449/177816
dc.language.isoeng
dc.relation.ispartofFungal Biology
dc.relation.ispartofsjr1,134
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCandida rugosa
dc.subjectDual-species biofilms
dc.subjectFungal biofilms
dc.subjectOral colonization
dc.subjectProstheses-related infections
dc.titleCandida/Candida biofilms. First description of dual-species Candida albicans/C. rugosa biofilmen
dc.typeArtigo
unesp.author.orcid0000-0002-6004-5951[2]

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
2-s2.0-84958581117.pdf
Tamanho:
1.44 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar

Coleções

Artigos - Análises Clínicas - FCFAR