DNA damage in peripheral blood mononuclear cells of patients undergoing anti-tuberculosis treatment

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dc.contributor.authorCardoso de Oliveira, Larissa Ragozo [UNESP]
dc.contributor.authorPeresi, Eliana [UNESP]
dc.contributor.authorTavares, Francilene Capel [UNESP]
dc.contributor.authorCorrêa, Camila Renata [UNESP]
dc.contributor.authorPierine, Damiana Tortolero [UNESP]
dc.contributor.authorCalvi, Sueli Aparecida [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:34:29Z
dc.date.available2014-05-20T13:34:29Z
dc.date.issued2012-08-30
dc.description.abstractTuberculosis (TB), a chronic infectious disease, is a major cause of morbidity and mortality worldwide. Expression of iNOS and consequent production of NO during the inflammatory process is an important defense mechanism against TB bacteria. We have tested whether pulmonary TB patients undergoing anti-tuberculosis treatment present DNA damage, and whether this damage is related to oxidative stress, by evaluating total hydrophilic antioxidant capacity and iNOS expression. DNA damage in peripheral blood mononuclear cells from patients and healthy tuberculin test (PPD) positive controls was evaluated by single-cell gel electrophoresis (comet assay), and iNOS expression was measured by qPCR. We also evaluated total hydrophilic antioxidant capacity in plasma from patients and controls. Compared to controls, pulmonary TB patients under treatment presented increased DNA damage, which diminished during treatment. Also, the antioxidant capacity of these individuals was increased at the start of treatment, and reduced during treatment. TB patients showed lower iNOS expression, but expression tended to increase during treatment. Our results indicate that pulmonary TB patients under anti-TB treatment exhibit elevated DNA damage in peripheral blood mononuclear cells. This damage was not related to nitric oxide but may be due to other free radicals. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.affiliationSão Paulo State Univ, Botucatu Med Sch UNESP, Dept Trop Dis, BR-18618000 São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Botucatu Med Sch UNESP, Dept Internal Med, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Botucatu Med Sch UNESP, Dept Trop Dis, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Botucatu Med Sch UNESP, Dept Internal Med, BR-18618000 São Paulo, Brazil
dc.format.extent82-85
dc.identifierhttp://dx.doi.org/10.1016/j.mrgentox.2012.04.003
dc.identifier.citationMutation Research-genetic Toxicology and Environmental Mutagenesis. Amsterdam: Elsevier B.V., v. 747, n. 1, p. 82-85, 2012.
dc.identifier.doi10.1016/j.mrgentox.2012.04.003
dc.identifier.issn1383-5718
dc.identifier.lattes2179450022699059
dc.identifier.urihttp://hdl.handle.net/11449/11823
dc.identifier.wosWOS:000306381400012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMutation Research: Genetic Toxicology and Environmental Mutagenesis
dc.relation.ispartofjcr1.996
dc.relation.ispartofsjr0,747
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectDNA damageen
dc.subjectTuberculosisen
dc.subjectAntioxidantsen
dc.subjectiNOSen
dc.titleDNA damage in peripheral blood mononuclear cells of patients undergoing anti-tuberculosis treatmenten
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes2179450022699059
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Doenças Tropicais e Diagnósticos por Imagem - FMB
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