TRPV4 activates autonomic and behavioural warmth-defence responses in Wistar rats

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dc.contributor.authorVizin, Robson Cristiano Lillo
dc.contributor.authorScarpellini, Carolina da S. [UNESP]
dc.contributor.authorIshikawa, Débora T.
dc.contributor.authorCorrea, Gabriela M. [UNESP]
dc.contributor.authorSouza, Cristiane Oliveira de
dc.contributor.authorBatalhão, Luciane Helena Gargaglioni [UNESP]
dc.contributor.authorCarrettiero, Daniel C.
dc.contributor.authorBicego, Kênia Cardoso [UNESP]
dc.contributor.authorAlmeida, Maria Camila
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionNational Institute of Science and Technology in Comparative Physiology
dc.date.accessioned2015-10-21T20:25:36Z
dc.date.available2015-10-21T20:25:36Z
dc.date.issued2015-06-01
dc.description.abstractAim In this study, we aimed at investigating the involvement of the warmth-sensitive channel - TRPV4 (in vitro sensitive to temperatures in the range of approx. 24-34 degrees C) - on the thermoregulatory mechanisms in rats.MethodsWe treated rats with a chemical selective agonist (RN-1747) and two antagonists (RN-1734 and HC-067047) of the TRPV4 channel and measured core body temperature, metabolism, heat loss index and preferred ambient temperature.ResultsOur data revealed that chemical activation of TRPV4 channels by topical application of RN-1747 on the skin leads to hypothermia and this effect was blocked by the pre-treatment with the selective antagonist of this channel. Intracerebroventricular treatment with RN-1747 did not cause hypothermia, indicating that the observed response was indeed due to activation of TRPV4 channels in the periphery. Intravenous blockade of this channel with HC-067047 caused an increase in core body temperature at ambient temperature of 26 and 30 degrees C, but not at 22 and 32 degrees C. At 26 degrees C, HC-067047-induced hyperthermia was accompanied by increase in oxygen consumption (an index of thermogenesis), while chemical stimulation of TRPV4 increased tail heat loss, indicating that these two autonomic thermoeffectors in the rat are modulated through TRPV4 channels. Furthermore, rats chemically stimulated with TRPV4 agonist choose colder ambient temperatures and cold-seeking behaviour after thermal stimulation (28-31 degrees C) was inhibited by TRPV4 antagonist.ConclusionOur results suggest, for the first time, that TRPV4 channel is involved in the recruitment of behavioural and autonomic warmth-defence responses to regulate core body temperature.en
dc.description.affiliationUniversidade Federal do ABC, Centro de Ciências Naturais e Humanas
dc.description.affiliationUnespSao Paulo State Univ, Dept Anim Morphol &Physiol, Coll Agr &Vet Sci, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Morfologia e Fisiologia Animal, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 470742/2010-0
dc.description.sponsorshipIdFAPESP: 2011/06528-1
dc.description.sponsorshipIdFAPESP: 2012/19966-0
dc.description.sponsorshipIdFAPESP: 2009/11446-4
dc.description.sponsorshipIdFAPESP: 2012/11836-0
dc.description.sponsorshipIdFAPESP: 2011/19131-2
dc.format.extent275-289
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1111/apha.12477/abstract;jsessionid=78335D14D0386D15F76F5B56FF0BFCAA.f02t04
dc.identifier.citationActa Physiologica. Hoboken: Wiley-blackwell, v. 214, n. 2, p. 275-289, 2015.
dc.identifier.doi10.1111/apha.12477
dc.identifier.issn1748-1708
dc.identifier.lattes5850453468994497
dc.identifier.urihttp://hdl.handle.net/11449/129130
dc.identifier.wosWOS:000354389100015
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofActa Physiologica
dc.relation.ispartofjcr5.930
dc.relation.ispartofsjr1,542
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectTail vasomotor toneen
dc.subjectThermogenesisen
dc.subjectThermopreferendumen
dc.subjectThermoregulationen
dc.subjectTRPV4 manipulationen
dc.subjectWarmth sensoren
dc.titleTRPV4 activates autonomic and behavioural warmth-defence responses in Wistar ratsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes5850453468994497[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Morfologia e Fisiologia Animal - FCAV