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Antibiofilm effect of cleaning agents for ocular prostheses
(2023-01-01) Andreotti, A. M. [UNESP]; Pereira de Caxias, F. [UNESP]; Alves de Sousa, C. [UNESP]; Freitas da Silva, E. V. [UNESP]; Silva, C. B. [UNESP]; Biléco, D. S. [UNESP]; Duque, C. [UNESP]; Goiato, M. C. [UNESP]; de Magalhães Bertoz, A. P. [UNESP]; dos Santos, D. M. [UNESP]; Universidade Estadual Paulista (UNESP)
Introduction. This study aimed to evaluate the antibiofilm effect of different agents (neutral soap, 4% chlorhexidine, Efferdent effervescent tablets, 1% triclosan, and citronella essential oil) used for ocular prosthesis cleaning. Material and Methods. Biofilms of S. aureus and S. epidermidis were formed on 60 ocular prosthesis acrylic resin specimens. The specimens were cleaned with the studied agents with different techniques. Microorganism counting was performed. Data were submitted to ANOVA and HSD Tukey-Kramer (p<.01). Results. When compared to the control group, all cleaning protocols promoted a reduction in growth of microorganisms. The 4% chlorhexidine, effervescent tablets, and 1% triclosan cleaning agents eliminated biofilm in all groups. Conclusion. Therefore, immersion in 4% chlorhexidine, effervescent tablets, and 1% triclosan could be the best protocols indicated for ocular prosthesis cleaning due to their ability to eliminate biofilm.
Early life stress exacerbates bone resorption and inhibits anxiety-like behaviour induced by apical periodontitis in rats
(2023-02-01) Bertolini Botelho, Maria Clara [UNESP]; Cintra, Luciano Tavares Angelo [UNESP]; da Silva, Cristiane Cantiga [UNESP]; Mitsuy Kayahara, Giseli [UNESP]; Belzunces Pereira, Rosani [UNESP]; Oliveira Santos, Mylena Fernanda [UNESP]; Issamu Miyahara, Glauco [UNESP]; Biasoli, Éder Ricardo [UNESP]; Penha Oliveira, Sandra Helena [UNESP]; Bernabé, Daniel Galera [UNESP]; Universidade Estadual Paulista (UNESP)
Aim: To evaluate the influence of the early life stress (ELS) on the severity of the apical periodontitis (AP) in Wistar rats. Methodology: Forty male Wistar rats were divided into four groups (n = 10): Control rats; AP—rats with AP; ELS—rats subject to ELS; AP + ELS—rats exposed to ELS and subject to AP. ELS was induced by maternal separation (MS) for a period of 3 h for 21 consecutive days. AP was induced via pulp exposure of the first and second right maxillary molars to the oral environment for 40 days. Three days before euthanasia, all rats underwent behavioural analysis to measure anxiety levels by elevated zero maze. Then, the rats were euthanized and the maxillas were removed to assess the occurrence and severity of AP. The periapical region was evaluated for the intensity of the inflammatory infiltrate and the extent of bone loss. The Mann–Whitney test was performed for nonparametric data, and the Tukey's or Student's t-test was performed for parametric data (p <.05). Results: The intensity of the inflammatory infiltrate was significantly larger in the AP + ELS group when compared with AP group (p <.05). The AP + ELS group exhibited significantly greater alveolar bone loss, with a periapical lesion size of 103.5 ± 29.88, compared with 72.3 ± 22.28 in the AP group (p <.05). Rats with AP displayed higher anxiety-like behaviour in relation to the control group (p <.05). However, exposure to ELS abolished the AP-induced increased anxiety-like ‘behaviour’ throughout, since that rats from AP + ELS group attended more the open arms than non-stressed rats with AP (p <.05). Conclusion: Early life stress is predictive of the severity of AP exacerbating the inflammatory process and increasing periapical bone resorption.
Reply to the opinion paper “The impact of stress on rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide: some theoretical concepts for scientific debate” by Silva et al.
(2023-02-01) Kayahara, Giseli Mitsuy [UNESP]; Verza, Flávia Alves [UNESP]; Valente, Vitor Bonetti [UNESP]; Miyahara, Glauco Issamu [UNESP]; Bernabé, Daniel Galera [UNESP]; Universidade Estadual Paulista (UNESP)
The Inflammation Level and a Microbiological Analysis of the Anophthalmic Cavities of Unilateral Ocular Prosthesis Users: A Blind, Randomized Observational Study
(2022-11-01) Penitente, Paulo Augusto [UNESP]; Da Silva, Emily Vivianne Freitas; Goiato, Marcelo Coelho [UNESP]; Maniçoba, Lorena Louise Pontes [UNESP]; Brito, Victor Gustavo Balera [UNESP]; Túrcio, Karina Helga Leal [UNESP]; Rodrigues, Alana Semenzin [UNESP]; Nagay, Bruna Egumi; Dos Santos, Daniela Micheline [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP); Universidade Estadual de Campinas (UNICAMP)
Irritation and biofilm adhesion are complaints associated with ocular prosthesis use. This study aimed to evaluate the effects of prosthesis repolishing on several conditions of anophthalmic volunteers. Participants were divided into two groups: intervention (IG, n = 10) and nonintervention (NIG, n = 6) groups. The anophthalmic cavity, contralateral eye, and prosthesis surface were evaluated at initial, day 15, and day 30 after repolishing. Microbiological analysis (colony-forming units), exfoliative cytology (conjunctiva inflammatory cells), sensory analysis (quantitative mechanical sensory test), tear production (Schirmer’s test), and conjunctival inflammation (clinical evaluation) were performed. Nonparametric tests were used to compare groups in the initial period and to analyze periods for the IG (p < 0.05). More microorganisms were formed in the anophthalmic socket and prosthesis than in the contralateral eye in the initial period. For IG, the anophthalmic cavity exhibited more microorganisms and inflammatory clinical signs in the initial period than at 15 and 30 after repolishing. The prosthesis showed greater accumulations of total bacteria and Candida albicans in the initial period than at 15 and 30 days after repolishing. The anophthalmic cavity had more palpebral inflammation than the contralateral eye. In conclusion, repolishing reduced the number of microorganisms and inflammatory signs over time.
Influence of different methods for classification of lymph node metastases on the survival of patients with oral squamous cell carcinoma
(2023-04-01) Tomo, Saygo [UNESP]; de Castro, Tamara Fernandes [UNESP]; Araújo, Winicius Arildo Ferreira [UNESP]; Collado, Francisco Urbano [UNESP]; Neto, Sebastião Conrado [UNESP]; Biasoli, Éder Ricardo [UNESP]; Bernabé, Daniel Galera [UNESP]; Miyahara, Glauco Issamu [UNESP]; Universidade Estadual Paulista (UNESP)
Background: Despite the advances in the classification of oral squamous cell carcinoma (OSCC) based on its extension by the TNM system, there is still a need for methods to better classify the patients to predict prognosis and indicate adjuvant therapy. Objectives: To analyze the influence of the number of positive lymph nodes (PN), lymph node ratio (LNR), and log odds of positive lymph nodes (LODDS) in survival of patients with OSCC. Methods: Clinicopathologic data from patients with OSCC who were treated with curative purposes by surgery and neck dissection (ND) with or without subsequent adjuvant therapies from 1991 to 2015 was retrospectively assessed. The impact of the PN, LNR, LODDS, and other variables on overall survival (OS) and disease-free survival (DFS) was analyzed in univariate and multivariate analyses. Results: One hundred nineteen patients were included in this study. In the univariate analysis the PN had a significant impact on OS (p = 0.001) and DFS (p = 0.020), and the LNR had a significant impact on the OS (p = 0.042). In the multivariate analysis with other relevant clinicopathologic variables, the PN was the only significantly independent factor influencing in the OS (p = 0.017) but not in DFS (p = 0.096). Conclusions: The PN is an independent prognostic indicator for OS and DFS in patients with OSCC and has the potential to aggregate the current AJCC classification. The LNR has potential to be an important prognostic indicator, but the methods for this classification require lapidation. The LODDS did not demonstrate prognostic potential.
Mantle cell lymphoma involving the oral and maxillofacial region: a study of 20 cases
(2023-01-01) Carvalho, Marcus Vinícius Ribeiro; Rodrigues-Fernandes, Carla Isabelly; de Cáceres, Cinthia Veronica Bardález López; Mesquita, Ricardo Alves; Martins, Manoela Domingues; Román Tager, Elena María José; Carlos, Roman; Pires, Fábio Ramôa; Robinson, Liam; van Heerden, Willie F.P.; Valente, Vitor Bonetti [UNESP]; Xavier-Júnior, José Cândido Caldeira; Bernabé, Daniel Galera [UNESP]; Pontes, Hélder Antônio Rebelo; Soares, Ciro Dantas; da Costa, Mauro Henrique Melo; Vargas, Pablo Agustin; Fonseca, Felipe Paiva; Universidade Estadual de Campinas (UNICAMP); Universidade Federal de Minas Gerais (UFMG); Federal University of Rio Grande do Sul; Centro Clínico de Cabeza y Cuello/Hospital Herrera Llerandi; Rio de Janeiro State University; University of Pretoria; Universidade Estadual Paulista (UNESP); Centro Universitário Católico Unisalesiano Auxilium; Universidade Federal do Pará (UFPA); Getúlio Sales Diagnósticos; Private practice
Objective: To investigate the clinicopathologic features of mantle cell lymphoma (MCL) involving the oral and maxillofacial region. Methods: The MCL cases were retrieved from the pathosis database of 6 pathology laboratories. Original hematoxylin and eosin slides and immunohistochemical reactions were reviewed for confirmation of the initial diagnosis. Clinical data of the cases were obtained from the patients’ pathosis and/or medical charts. Results: Twenty cases were included in the study, showing a male predominance and a mean age of 66 years. The oral cavity (12 cases) and the oropharynx (5 cases) were the most commonly involved subsites. Most cases presented as asymptomatic swellings, with 2 cases showing bilateral involvement of the palate. The classic histologic variant predominated (12/20 cases). All cases expressed CD20 with nuclear cyclin D1 positivity. SOX11 was seen in 9/13 cases, CD5 in 6/16 cases, Bcl2 in 16/19 cases, CD10 in 2/20 cases, and Bcl6 in 4/16 cases. Ki67 showed a mean proliferation index of 40.6%. The Epstein-Barr virus (EBV) was negative in all cases investigated. Follow-up data was available for 7 patients, with 5 currently alive and 2 deceased. Conclusion: Mantle cell lymphoma, albeit rare, may manifest in the oral and maxillofacial region. Its histologic heterogeneity demands a high degree of diagnostic skill from pathologists.
Predisposing factors for increased cortisol levels in oral cancer patients
(2022-02-01) Figueira, Jéssica Araújo [UNESP]; Sarafim-Silva, Bruna Amélia Moreira [UNESP]; Gonçalves, Gislene Maria [UNESP]; Aranha, Laerte Nivaldo; Lopes, Flávia Lombardi [UNESP]; Corrente, José Eduardo [UNESP]; Biasoli, Éder Ricardo [UNESP]; Miyahara, Glauco Issamu [UNESP]; Bernabé, Daniel Galera [UNESP]; Universidade Estadual Paulista (UNESP); Sabin Laboratory
Cancer patients may have a dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis and abnormal secretion of cortisol. Increased cortisol levels have been associated with worse prognosis in patients with different types of tumors. Although anxiety and depression can trigger an abnormal cortisol secretion, little is known regarding the influence of these emotional disorders on HPA axis dysregulation in cancer patients when evaluating together with demographic, clinicopathological and biobehavioral variables. This cross-sectional study analyzed the pre-treatment plasma cortisol levels of 133 patients with oral squamous cell carcinoma (OSCC) and its association with demographic, clinicopathological, biobehavioral and psychological variables. Plasma cortisol levels were measured by electrochemiluminescence, and anxiety and depression symptoms were assessed using Beck Anxiety Inventory (BAI) and Depression (BDI), respectively. Demographic, clinicopathological and biobehavioral data were collected from patients' medical records. Results from multivariate analysis showed that the occurrence of cancer-induced pain was predictive for higher cortisol levels (OR = 5.388, p = 0.003). Men with OSCC were 4.5 times more likely to have higher plasma cortisol levels than women (OR = 4.472, p = 0.018). The effect of sex on cortisol concentrations was lost in the adjusted model for clinical staging (OR = 2.945, p = 0.116). The absence of chronic alcohol consumption history was a protective factor for highest hormone concentrations in oral cancer patients (OR = 0.104, p = 0.004). Anxiety symptoms measured by BAI as “hands trembling” (OR = 0.192, p = 0.016) and being “nervous” (OR = 0.207, p = 0.0004) were associated with lower cortisol levels. In contrast, the feeling of “fear of losing control” was a risk factor for highest hormone concentrations (OR = 6.508, p = 0.0004). The global score and specific symptoms of depression measured by the BDI were not predictive for plasma hormone levels (p > 0.05). Together, our results show that pain, alcohol consumption and feeling fear are independent factors for increased systemic cortisol levels in patients with oral cancer. Therefore, psychological intervention, as well as control of pain and alcohol consumption, should be considered to prevent the negative effects of cortisol secretion dysregulation in cancer patients.
Potential of lymph-node ratio as a prognostic factor for patients with oral squamous cell carcinoma
(2022-01-01) Tomo, Saygo [UNESP]; Araújo, Winicius Arildo Ferreira [UNESP]; de Castro, Tamara Fernandes [UNESP]; Neto, Sebastião Conrado [UNESP]; Collado, Francisco Urbano [UNESP]; Biasoli, Éder Ricardo [UNESP]; Bernabé, Daniel Galera [UNESP]; Miyahara, Glauco Issamu [UNESP]; Universidade Estadual Paulista (UNESP)
Background: To analyze the influence of the lymph node ratio (LNR) in survival of patients with OSCC Methods: Clinicopathologic data from patients with OSCC who were treated with curative surgery and neck dissection (ND) with or without adjuvant therapies from 1991 to 2015 was retrospectively assessed. The impact of LNR and other variables on overall survival (OS) and disease-free survival (DFS) was analyzed in univariate and multivariate analyses. Results: One hundred nineteen patients were included. In the univariate analysis the LNR had a significant impact on OS (p = 0.01) and DFS (p = 0.01). In the multivariate analysis, the LNR was the only significantly independent factor influencing in the OS (p = 0.03). The adjuvant therapies did not influence on the OS (p = 0.42) and DFS (p = 0.10). Conclusions: The LNR is an independent prognostic factor in patients with OSCC. The LNR alone is not recommended to indicate the performance of adjuvant therapies.
Simultaneous occurrence of melanocytic nevus and melanotic macule in the vermilion of the lower lip
(2022-01-01) Silva Filho, Nilton José da [UNESP]; de Abreu, Lukas Mendes [UNESP]; de Melo Cardoso, Diovana [UNESP]; Valente, Vitor Bonetti [UNESP]; Xavier-Júnior, José Cândido Caldeira; Miyahara, Glauco Issamu [UNESP]; Biasoli, Éder Ricardo [UNESP]; Bernabé, Daniel Galera [UNESP]; Universidade Estadual Paulista (UNESP); Centro Universitário Católico Unisalesiano Auxilium
Diagnosis of oral pigmented lesions in the oral cavity can be very challenging. While melanotic macule is considered the most frequent oral pigmented lesion, melanocytic nevi are uncommonly observed. This report describes an uncommon case of a young woman with simultaneous occurrence of oral melanocytic nevus (OMN) and oral melanotic macule (OMM) in the vermilion of the lower lip. The etiopathogenesis, histopathological characteristics and clinical management of these two pigmented oral lesions are discussed.
Relationship of excessive daytime sleepiness with bruxism, depression, anxiety, stress, and sex in odontology students – A cross sectional study
(2022-06-01) Turcio, Karina-Helga [UNESP]; de Moraes-Melo-Neto, Clóvis-Lamartine [UNESP]; de Caxias, Fernanda-Pereira [UNESP]; Goiato, Marcelo-Coelho [UNESP]; dos Santos, Daniela-Micheline [UNESP]; Januzzi, Marcella-Santos [UNESP]; Guiotti, Aimée-Maria [UNESP]; Bertoz, André-Pinheiro-de Magalhães [UNESP]; Brandini, Daniela-Atili [UNESP]; Universidade Estadual Paulista (UNESP)
Background: To verify if there is a correlation of excessive daytime sleepiness (EDS) with bruxism, depression, anxiety, stress, and sex in odontology students. Material and Methods: Four hundred dental students were selected. Students included in the study were those that were healthy, over 18 years old, and with similar weekly academic activities. Students excluded were those with temporomandibular disfunction, a high possibility of possessing obstructive sleep apnea and illnesses that cause EDS; along with smokers, users of illicit drugs, users of psychiatric medication, and those that abuse the consumption of alcohol. After the application of these criteria, 128 students were included in this study. EDS, bruxism, depression, anxiety, and stress were evaluated by the Epworth Sleepiness Scale, the Pintado et al. questionnaire, Beck Depression Inventory, Beck Anxiety Inventory, and the LIPP Adult Stress Symptom Inventory. Afterwards, the Spearman test (p< 0.05) was applied. Results: A high probability of EDS was present in 33.6% of the students. There was a positive correlation of EDS with females (Rank Correlation= 0.209; p= 0.018) and depression (Rank Correlation= 0.174; p= 0.049); between females and stress (Rank Correlation= 0.199; p= 0.024), and between females and anxiety (Rank Correlation= 0.178; p= 0.045). There was a positive correlation of bruxism with anxiety (Rank Correlation= 0.255; p= 0.004) and stress (Rank Correlation= 0.201; p= 0.023). There was no correlation of EDS with bruxism (p= 0.354), stress (p= 0.277), or anxiety (p= 0.114). There was no correlation of bruxism with females (p= 0.082) or depression (p= 0.362). Conclusions: A high probability of EDS was present in 33.6% of dentistry students, and there was a positive correlation of EDS with females and depression
Relationship of bruxism with oral health-related quality of life and facial muscle pain in dentate individuals
(2022-01-01) Turcio, Karina-Helga-Leal [UNESP]; Melo Neto, Clóvis-Lamartine de Moraes [UNESP]; Pirovani, Beatriz-Ommati [UNESP]; Santos, Daniela-Micheline dos [UNESP]; Guiotti, Aimée-Maria [UNESP]; Bertoz, André-Pinheiro de Magalhães [UNESP]; Brandini, Daniela-Atili [UNESP]; Universidade Estadual Paulista (UNESP)
Background: To determine whether there is a correlation of bruxism (sleep, daytime, or both) with oral health-related quality of life and facial pain of muscular origin in dentate individuals. Materials and Methods: Seventy-four dentate patients (complete dentition) were included in this study. These individuals had pain in the facial muscles due to temporomandibular disorder (TMD). Smokers; and those with obstructive sleep apnea, TMD of joint origin associated or not with pain, malocclusion, and cancer; and users of illicit drugs, psychiatric medications, and alcohol were excluded. Obstructive sleep apnea, bruxism (of sleep and/or daytime), facial muscle pain, and oral health-related quality of life were assessed by the following questionnaires: Berlin Questionnaire, Pintado et al. questionnaire, VAS (Visual Analog Scale) facial muscle pain questionnaire, and Oral Health Impact Profile – 14. Four groups were created: 1) no bruxism; 2) sleep bruxism; 3) daytime bruxism; and 4) sleep and daytime bruxism. Spearman’s correlation test was applied to verify if there was a correlation between the collected data. P values less than 0.05 were considered statistically significant. Results: There was a positive correlation of daytime bruxism with mean pain in the last 3 months (P<0.05) and the worst pain experienced in the last 3 months (P<0.05). Conclusions: Bruxism (sleep, daytime, or both) showed a positive correlation with lower oral health-related quality of life (P<0.05)
Encephalopathy responsive to thiamine in severe COVID-19 patients
(2021-07-01) Oliveira, Marcus Vinicius Branco de; Irikura, Sergio; Lourenço, Fabiani Honorato de Barros; Shinsato, Monica; Irikura, Tereza Cristina Duarte Batista; Irikura, Rodrigo Batista; Albuquerque, Tales Vieira Cavalvanti; Shinsato, Vilma Neri; Orsatti, Vinicius Nakad; Fontanelli, Antônio Mendes; Samegima, Danyelle Amélia Grecco; Gonçalves, Marcus Vinícius Magno; Bernabé, Daniel Galera [UNESP]; Unimed Hospital of Araçatuba; University of the Region of Joinville (UNIVILLE); Universidade Estadual Paulista (UNESP)
Encephalopathy is one of the most frequent neurological complications of severe Coronavirus Disease 2019 (COVID-19) patients. Cytokine storm and sepsis, hypercatabolic states, the use of furosemide and dialytic therapy represent risk factors for thiamine deficiency and are also found in patients with severe COVID-19. In this retrospective case series, we report clinical and neurological findings of fifteen patients with COVID-19-associated Wernicke Encephalopathy (WE) and their response to treatment with intravenous thiamine. All patients had encephalopathy, with 67% displaying at least one additional sign of classic WE triad (ophthalmoparesis and ataxia). Two patients (13%) had the classic triad. All COVID-19 patients had significant improvement of the neurological manifestations between two to five days after intravenous thiamine administration. Eleven patients (73%) had good neurological outcome at hospital discharge and only two patients (13%) died. This case series suggests that thiamine deficiency may be an etiology of encephalopathy in severe COVID-19 patients and its treatment may represent a safety and low-cost response to reduce the neurological burden.
Interventions for Radiation-Induced Fibrosis in Patients With Breast Cancer: Systematic Review and Meta-analyses
(2022-05-01) Nogueira, Regiane Mazzarioli Pereira [UNESP]; Vital, Flávia Maria Ribeiro; Bernabé, Daniel Galera [UNESP]; Carvalho, Marcos Brasilino de; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP); Pontifical Catholic University of Rio de Janeiro; Hospital Heliópolis
Purpose: Radiation therapy can affect normal tissues in patients with breast cancer, causing adverse effects such as fibrosis. Although there are several interventions for radiation-induced fibrosis, the efficacy of these procedures is still unclear. The purpose of this review is to evaluate the efficacy of interventions for radiation-induced fibrosis in patients with breast cancer. Methods and Materials: This is a systematic review of randomized clinical trials. Studies that compared any intervention for fibrosis to another intervention, placebo, or no intervention were included. Outcomes assessed were fibrosis, adverse events, quality of life, treatment adherence, pain, and functionality. Results: A total of 2501 publications were found, and 7 studies were selected because they met the inclusion criteria. The interventions for fibrosis were pentoxifylline and vitamin E, grape seed extract, kinesiotherapy, and endermotherapy. The results showed great heterogeneity in the treatment protocols for radiation-induced fibrosis in patients with breast cancer and in their evaluation metrics. The meta-analyses showed no benefit in using pentoxifylline and vitamin E compared with placebo or no intervention (standardized mean difference: −0.30; 95% confidence interval, −0.79 to 0.20; P = .24 [very low evidence]) compared with placebo and vitamin E (standardized mean difference: −0.09; 95% confidence interval, −0.66 to 0.49; P = .77 [moderate evidence]), respectively, assessed by the Late Effects Normal Tissue Task Force–Subjective, Objective, Management, and Analytic (LENT-SOMA) scoring scale. Conclusions: The effectiveness of these interventions for the treatment of radiation-induced fibrosis in patients with breast cancer could not be determined. Although isolated studies show significant results favorable to the experimental groups, caution should be exercised in these findings because of the small number, small sample size, and high risk of bias presented by some of the included studies, which makes the recommendation for clinical practice still weak.
Stress hormone norepinephrine incites resistance of oral cancer cells to chemotherapy
(2022-03-05) Tjioe, Kellen Cristine [UNESP]; Cardoso, Diovana Melo [UNESP]; Oliveira, Sandra Helena Penha [UNESP]; Bernabé, Daniel Galera [UNESP]; Universidade Estadual Paulista (UNESP)
This study investigated whether norepinephrine (NE) and epinephrine (E) interfere in the response of head and neck squamous cell carcinoma (SCC) cell lines to cisplatin and explored the mechanisms of chemoresistance. Head and neck SCC-derived cell lines SCC-9, Cal27, SCC-25, and FaDu were stimulated with NE or E and treated with the inhibitory concentration of cisplatin for 24 h. As for adrenergic receptors (ADRB) inhibition, cells were treated with propranolol. The results showed that, when combined with NE, cisplatin effectiveness against SCC-9 and Cal27 but not SCC-25 and FaDu cells were notably reduced. E did not affect the response of the cells to cisplatin. Further experiments were performed with the responsive SCC-9 and SCC-25 cell lines and the hormone NE. The time course assay showed that stimulation of oral SCC cells with NE decreased the cleavage of caspase-3 and expression of multidrug resistance protein 1 (MDR-1) but only transiently affected ATP-binding cassette (ABC) subfamily G, isoform 2 protein (ABCG2) expression. The expression of cleaved caspase-3 and Bcl-2 were, respectively, decreased and increased by the combination of NE and cisplatin in SCC-9 and Cal27 cells. NE-induced resistance was reverted by previous treatment with propranolol. Expressions of ABCG2, and p-Akt but not of MDR-1, were enhanced by NE plus cisplatin when compared to cisplatin only in both cell lines. Migratory activity of oral SCC cells challenged with cisplatin was not affected by NE. These findings reveal for the first time that stress hormone NE induces resistance of oral cancer cells to cisplatin in vitro through the ADRB/Akt/ABCG2 pathway, pumping the drug out of the cell and inhibiting apoptosis.
A submucous yellow nodule of the fornix
(2022-01-01) Miyahara, Glauco Issamu [UNESP]; de Castro, Tamara Fernandes [UNESP]; Araújo, Winícius Arildo Ferreira [UNESP]; Tomo, Saygo [UNESP]; Biasoli, Eder Ricardo [UNESP]; Santos-Silva, Alan Roger; Xavier-Júnior, José Cândido Caldeira; Crivelini, Marcelo Macedo [UNESP]; Bernabé, Daniel Galera [UNESP]; Universidade Estadual Paulista (UNESP); Universidade Estadual de Campinas (UNICAMP); Pathology Institute of Araçatuba; Centro Universitário Católico Unisalesiano Auxilium
Clinicopathological and prognostic profile of non-smoking and non-drinking head and neck cancer patients: a population-based comparative study
(2022-04-01) Bonetti Valente, Vitor [UNESP]; Mantovan Mazzon, Bruna [UNESP]; Urbano Collado, Francisco [UNESP]; Conrado Neto, Sebastião [UNESP]; Lúcia Marçal Mazza Sundefeld, Maria [UNESP]; Ricardo Biasoli, Éder [UNESP]; Issamu Miyahara, Glauco [UNESP]; Galera Bernabé, Daniel [UNESP]; Universidade Estadual Paulista (UNESP)
Tobacco and alcohol consumption are the main risk factors for head and neck cancer (HNC). However, some individuals do not have a history of addiction and even so they develop the disease. This study evaluated clinicopathological and prognostic features of non-smoking and non-drinking (NSND) HNC patients. A sample of smoking and/or drinking (SD) HNC patients was used to explore the differences with NSND patients. Data were obtained from 667 patients with squamous cell carcinoma in oral cavity, oropharynx or larynx. The group of NSND individuals represented 12.6% of the HNC patients and had higher prevalence of women and elderly compared to SD group. Among NSND patients, most of them displayed tumors in oral cavity, advanced clinical staging and absence of second primary tumor. NSND HNC patients had higher tumor recurrence and increased overall and specific survival rates at 5-years follow-up. The NSND group showed higher prevalence of tumors in lips and alveolar ridge, lower rate of tumors in floor of mouth and no tumor in soft palate. NSND patients show a distinct profile from that found in SD patients. Clinicopathological features from NSND patients should be considered for HNC management.
Focal epithelial hyperplasia (Heck's disease) in Brazilian indigenous children
(2022-01-01) Simonato, Luciana Estevam; de Arruda, Farid Jamil Silva; Marques, Taís Cristina Nascimento; Miyahara, Glauco Issamu [UNESP]; Tomo, Saygo; University Brasil; Universidade Estadual Paulista (UNESP); AC Camargo Cancer Center
Propranolol inhibits cell viability and expression of the pro-tumorigenic proteins Akt, NF-ĸB, and VEGF in oral squamous cell carcinoma
(2022-04-01) Shibuya, Caroline Miho [UNESP]; Tjioe, Kellen Cristine [UNESP]; Oliveira, Sandra Helena Penha [UNESP]; Bernabé, Daniel Galera [UNESP]; Universidade Estadual Paulista (UNESP)
Background: Propranolol (PPL) has been suggested as an option for the treatment of various types of cancer. However, data regarding its effectiveness against oral cancer are scarce. Thus, we aimed to evaluate the antitumor potential of PPL in oral squamous cell carcinoma (OSCC) in vitro. Methods: OSCC cell lines, SCC-9, SCC-25, and Cal27, were treated with PPL at different times and concentrations. OSCC cells were treated with PPL alone or in combination with cisplatin (CDDP) or 5-fluorouracil (5-FU). Cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of phosphorylated (p)-Akt, p-S6, p-PTEN, p-P65, and VEGF was verified by immunofluorescence. The migratory activity of OSCC cells was evaluated using a wound-healing assay. Results: PPL reduced OSCC cell viability in a dose- and time-dependent manner. Concentrations above 300 µM, 110 µM, and 100 µM for SCC-9, Cal27, and SCC-25, respectively, significantly eliminated tumor cells. The combination of PPL with CDDP and 5-FU enhanced their antitumor effects. There was a modest difference between the use of the IC30 and IC50 of PPL in the combinatory options. PPL downregulated p-P65 NF-ĸB and VEGF expression in SCC-9 and Cal27 cells but not in SCC-25 cells. PPL inhibited the phosphorylation of Akt and s6 and increased the phosphorylation of PTEN in all OSCC cell lines studied. PPL inhibited OSCC cell migration after 24 h of treatment. Conclusion: PPL was effective against oral cancer cells and enhanced standard-of-care. PPL inhibited cell viability and the expression of pAkt, NF-ĸB, and VEGF.
Garlic burn trauma of the oral mucosa in a patient with trigeminal neuralgia: A case report
(2022-01-01) Tomo, Saygo; Santos, Ingrid da Silva; Cruz, Thiago Moreira da; Miyahara, Glauco Issamu [UNESP]; Simonato, Luciana Estevam; AC Camargo Cancer Center; Federal University of Rio Grande do Sul; University Brasil; Universidade Estadual Paulista (UNESP); Center of Dental Specialties
Garlic is one of the most common medicinal plants used since ancient times to treat diverse diseases within several cultures worldwide. Nevertheless, this plant has the potential to cause chemical burns of the skin and mucosa. This report presents a case of a garlic burn in the palate of a 57-year-old woman who applied garlic to the palate to relieve the pain of trigeminal neuralgia. This case demonstrates the potential of garlic to cause chemical burns to the oral mucosa and raises an alert to oral health professionals regarding inappropriate self-treatment methods.
HIV-related plasmablastic lymphoma causing extensive bone destruction in the mandible
(2022-03-01) Kayahara, Giseli Mitsuy [UNESP]; Valente, Vitor Bonetti [UNESP]; Salzedas, Leda Maria Pescinini [UNESP]; Passador-Santos, Fabrício; Furuse, Cristiane [UNESP]; Biasoli, Éder Ricardo [UNESP]; Miyahara, Glauco Issamu [UNESP]; Bernabé, Daniel Galera [UNESP]; Universidade Estadual Paulista (UNESP); São Leopoldo Mandic Research Institute
Plasmablastic lymphoma (PBL) is a rare subtype of large B-cell lymphoma commonly associated with HIV infection. HIV-related PBL has a dismal prognosis. The aggressive clinical course of the disease may lead to the development of rapid-growing swellings, like several benign and malignant conditions. Herein, we reported the case of a 38-year-old woman with a painful swelling in the mandible initially diagnosed as an abscess derived from a tooth extraction. Intraoral examination revealed a painful swelling with reddish, white and purplish areas in the posterior region of the mandible without signs of infection. Imaging exams showed an extensive bone destruction in the left mandibular body. Histopathological examination revealed a high proliferation of plasmacytoid cells with nuclear hyperchromatism. Tumor cells were negative for CD20, and positive for Ki-67, CD138, IgG and lambda chain. The diagnosis of oral PBL was defined and serological test showed positivity for HIV. Eight months after starting treatment, the patient died due to complications of cancer treatment. Lymphoproliferative malignancies related to HIV infection should be included in the differential diagnosis of rapid-growing swellings in the oral cavity.

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