Publicação: Fibroblast contributes to osteoblast differentiation in a sonic hedgehog signaling-independent manner
Carregando...
Arquivos
Data
Autores
Orientador
Zambuzzi, Willian Fernando 
Coorientador
Pós-graduação
Curso de graduação
Ciências Biológicas - IBB
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Tipo
Trabalho de conclusão de curso
Direito de acesso
Acesso aberto
Resumo
Resumo (inglês)
We hypothesized that communication between osteoblast and fibroblasts (FB) contributes to bone dynamic tissue. We harvested cell-free supernatants from fibroblast to treat osteoblast for testing viability and differentiation mechanisms, mainly monitoring the involvement of Shh signaling in this paracrine mechanism. By exploring immunoblotting approach we have shown that FB-released factors interfere on osteoblast metabolism by up-regulating the phosphorylation of FAK and Rac-1 proteins at the early stage and later contributing with osteoblast differentiation by upmodulating alkaline phosphatase (ALP) and in vitro mineralization. During osteoblast differentiation process, we also showed that Shh signaling proteins weren't upmodulated in disagreement with the osteogenic medium (O.M.) response, where Shh, Patched and Gli-1 were up-regulated. Altogether, our results show for the first time a possible mechanism involved on the crosstalk between fibroblast and osteoblast, since it is possible to observe that factors released from fibroblasts interfere on osteoblast metabolism at a Shh-independent manner. In this sense, we believe these results help us to understand the importance of the paracrine metabolism through bone resident cells for supporting bone dynamic biology, opening new avenues for comprehending bone diseases etiologies, as the case of osteoporosis
Resumo (português)
Não disponível
Descrição
Palavras-chave
Ossos, Proteínas, Fibroblastos, Hedgehog proteins
Idioma
Inglês
Como citar
FERNANDES, Célio Junior da Costa. Fibroblast contributes to osteoblast differentiation in a sonic hedgehog signaling-independent manner. 2017. 1 CD-ROM. Trabalho de conclusão de curso (bacharelado - Ciências Biológicas) - Universidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Biociências, 2017.
