Differential expression of a retrotransposable element, Rex6, in Colossoma macropomum fish from different Amazonian environments
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Coadvisor
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Undergraduate course
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Mobile Genetic Elements
Type
Article
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Acesso restrito
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Abstract
Transposable elements (TEs) are DNA sequences that have the ability to move and replicate within the genomes. TEs can be classified according to their intermediates of transposition, RNA (retrotransposons) or DNA. In some aquatic organisms, it has been observed that environmental factors such as pH, temperature and pollution may stimulate differential transcription and mobilization of retrotransposons. In light of this information, the present study sought to evaluate the expression of Rex6 TE transcripts in Colossoma macropomum, which is a very commercially exploited fish in Brazil. In order to establish a comparative analysis using real-time PCR, the samples were collected from Amazonian rivers with different physical and chemical characteristics (distinguished by clear water and black water). Quantitative RT-PCR analyses revealed a differential pattern of expression between tissues collected from different types of water (clear and black waters). When it came to the hepatic and muscle tissues sampled, the levels of Rex6 transcripts were significantly different between the two Amazonian water types. These results suggest that environmental conditions operate differently in the regulation of Rex6 transcription in C. macropomum, results which have implications in the reshaping of the genome against environmental variations.
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Keywords
Differential expression, Quantitative rt-pcr, Transcript tissue distribution, Transposable elements
Language
English
Citation
Mobile Genetic Elements, v. 4, p. 1-4, 2014.
