Typing class I HLA-A gene using a nested PCR-RFLP procedure
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Undergraduate course
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Associação Brasileira de Divulgação Científica (ABRADIC)
FapUNIFESP (SciELO)
FapUNIFESP (SciELO)
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Abstract
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
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Language
English
Citation
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 6, p. 837-842, 2005.
