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Crystallization and preliminary X-ray crystallographic analysis of chorismate synthase from Mycobacterium tuberculosis

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dc.contributor.authorDias, MVB
dc.contributor.authorEly, F.
dc.contributor.authorCanduri, F.
dc.contributor.authorPereira, J. F.
dc.contributor.authorBasso, L. A.
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorde Azevedo, W. F.
dc.contributor.authorSantos, D. S.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Rio Grande do Sul (UFRGS)
dc.contributor.institutionPontificia Univ Catolica Rio Grande do Sul
dc.date.accessioned2014-05-20T13:54:20Z
dc.date.available2014-05-20T13:54:20Z
dc.date.issued2004-11-01
dc.description.abstractThe enzymes of the shikimate pathway are potential targets for the development of new therapies because they are essential for bacteria but absent from mammals. The last step in this pathway is performed by chorismate synthase (CS), which catalyzes the conversion of 5-enolpyruvylshikimate-3-phosphate to chorismate. Optimization of crystallization trials allowed the crystallization of homogeneous recombinant CS from Mycobacterium tuberculosis (MtCS). The crystals of MtCS belong to space group P6(4)22 (or P6(2)22) and diffract to 2.8 Angstrom resolution, with unit-cell parameters a = b = 129.7, c = 156.8 Angstrom. There are two molecules in the asymmetric unit. Molecular-replacement trials were not sucessful. Heavy-atom derivative screening is in progress.en
dc.description.affiliationUNESP, Dept Fis, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUFRGS, Dept Biol Mol & Biotecnol, Rede Brasileira Pesquisas TB Grp Microbiol Mol &, BR-91501970 Porto Alegre, RS, Brazil
dc.description.affiliationUNESP, Inst Biosci, Dept Biol, CEIS,Lab Struct Biol & Zoochem, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationPontificia Univ Catolica Rio Grande do Sul, Inst Pesquisas Biomed, Ctr Pesquisas Biol Mol & Func, BR-90619900 Porto Alegre, RS, Brazil
dc.description.affiliationUnespUNESP, Dept Fis, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biosci, Dept Biol, CEIS,Lab Struct Biol & Zoochem, BR-13506900 Rio Claro, SP, Brazil
dc.format.extent2003-2005
dc.identifierhttp://dx.doi.org/10.1107/S0907444904019869
dc.identifier.citationActa Crystallographica Section D-biological Crystallography. Copenhagen: Blackwell Munksgaard, v. 60, p. 2003-2005, 2004.
dc.identifier.doi10.1107/S0907444904019869
dc.identifier.issn0907-4449
dc.identifier.lattes2901888624506535
dc.identifier.urihttp://hdl.handle.net/11449/19411
dc.identifier.wosWOS:000224595200012
dc.language.isoeng
dc.publisherBlackwell Munksgaard
dc.relation.ispartofActa Crystallographica Section D: Biological Crystallography
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleCrystallization and preliminary X-ray crystallographic analysis of chorismate synthase from Mycobacterium tuberculosisen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderBlackwell Munksgaard
dspace.entity.typePublication
unesp.author.lattes2901888624506535
unesp.author.orcid0000-0002-5312-0191[1]
unesp.author.orcid0000-0002-2866-5704[2]
unesp.author.orcid0000-0002-7363-8211[6]
unesp.author.orcid0000-0003-0903-2407[5]
unesp.author.orcid0000-0003-4971-463X[8]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentBiologia - IBpt
unesp.departmentFísica - IBILCEpt

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Coleções

São José do Rio Preto - IBILCE - Instituto de Biociências, Letras e Ciências Exatas
Rio Claro - IB - Instituto de Biociências