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Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy

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dc.contributor.authorLima, Ana Silvia Gouvêa
dc.contributor.authorGuidelli, Ana Maria [UNESP]
dc.contributor.authorAbreu, Irlan Leite de [UNESP]
dc.contributor.authorLemos, Manuel Victor Franco [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:13:09Z
dc.date.available2014-05-20T13:13:09Z
dc.date.issued2002-01-01
dc.description.abstractPCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.en
dc.description.affiliationUSP Departamento de Genética
dc.description.affiliationUNESP Departamento de Biologia Aplicada à Agropecuária
dc.description.affiliationUnespUNESP Departamento de Biologia Aplicada à Agropecuária
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent225-229
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572002000200017
dc.identifier.citationGenetics and Molecular Biology. Sociedade Brasileira de Genética, v. 25, n. 2, p. 225-229, 2002.
dc.identifier.doi10.1590/S1415-47572002000200017
dc.identifier.fileS1415-47572002000200017.pdf
dc.identifier.issn1415-4757
dc.identifier.lattes7179273060624761
dc.identifier.scieloS1415-47572002000200017
dc.identifier.scopus2-s2.0-0036620718
dc.identifier.urihttp://hdl.handle.net/11449/1029
dc.language.isoeng
dc.publisherSociedade Brasileira de Genética
dc.relation.ispartofGenetics and Molecular Biology
dc.relation.ispartofjcr1.493
dc.relation.ispartofsjr0,638
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectREPen
dc.subjectERICen
dc.subjectcrystal proteinen
dc.subjectparasporal bodiesen
dc.titleIdentification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopyen
dc.typeArtigo
dspace.entity.typePublication
unesp.author.lattes7179273060624761

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