Publicação: Integrated analysis of halogenated organic pollutants in sub-millilitre volumes of venous and umbilical cord blood sera
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Data
2010-03-01
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Coorientador
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Resumo
A rapid, robust and economical method for the analysis of persistent halogenated organic compounds in small volumes of human serum and umbilical cord blood is described. The pollutants studied cover a broad range of molecules of contemporary epidemiological and legislative concern, including polychlorobiphenyls (PCBs), polychlorobenzenes (CBs), hexachlorocyclohexanes (HCHs), DDTs, polychlorostyrenes (PCSs) and polybromodiphenyl ethers (PBDEs). Extraction and clean-up with n-hexane and concentrated sulphuric acid was followed with analysis by gas chromatography coupled to electron capture (GC-ECD) and GC coupled to negative ion chemical ionisation mass spectrometry (GC-NICI-MS). The advantages of this method rest in the broad range of analytes and its simplicity and robustness, while the use of concentrated sulphuric acid extraction/clean-up destroys viruses that may be present in the samples. Small volumes of reference serum between 50 and 1000 μL were extracted and the limits of detection/quantification and repeatability were determined. Recoveries of spiked compounds for the extraction of small volumes (≥300 μL) of the spiked reference serum were between 90% and 120%. The coefficients of variation of repeatability ranged from 0.1-14%, depending on the compound. Samples of 4-year-old serum and umbilical cord blood (n = 73 and 40, respectively) from a population inhabiting a village near a chloro-alkali plant were screened for the above-mentioned halogenated pollutants using this method and the results are briefly described. © 2010 Springer-Verlag.
Descrição
Palavras-chave
Blood serum, Endocrine disruptors, GC-ECD, GC-MS, Pesticides, Trace halogenated organic compounds, Umbilical cord serum, Venous serum, Endocrine disruptor, Halogenated organic compounds, Umbilical cords, Alkalinity, Blood, Electrochemical sensors, Electrochromic devices, Ethers, Gas chromatography, Halogenation, Hexane, Mass spectrometry, Polychlorinated biphenyls, Reduction, Sulfuric acid, Viruses, Organic pollutants, halogenated hydrocarbon, blood, chemistry, electrospray mass spectrometry, evaluation, female, fetus blood, gas chromatography, human, male, methodology, pollutant, preschool child, serum, statistical parameters, Child, Preschool, Chromatography, Gas, Environmental Pollutants, Female, Fetal Blood, Humans, Hydrocarbons, Halogenated, Limit of Detection, Male, Serum, Spectrometry, Mass, Electrospray Ionization
Idioma
Inglês
Como citar
Analytical and Bioanalytical Chemistry, v. 396, n. 6, p. 2265-2272, 2010.
