Mouse extensor digitorum longus and soleus show distinctive ultrastructural changes induced by veratrine
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We investigated whether veratrine (5 μl, 10 ng/kg) injected into the mouse extensor digitorum longus (EDL) (fast-twitch) and soleus (SOL) (slow-twitch) muscles provokes distinctive ultrastructural disturbances 15, 30 and 60 min later. The mitochondria in SOL were affected earlier (within 15 min) than in EDL. Swelling of the sarcoplasmic reticulum terminal cisternae was more marked in EDL than in SOL and caused distortion of sarcomeres so that fragmentation of myofilaments was more pronounced in EDL. Hypercontracted sarcomeres were seen mainly in SOL and veratrine caused infoldings of the sarcolemma only in this muscle. In both muscles, the T-tubules remained unaffected and by 60 min after veratrine most of the above alterations had reverted to normal. Pretreatment with tetrodotoxin prevented the alterations induced by veratrine. This suggests that most of the alterations resulted from the enhanced influx of Na+ into muscle fibers. These results emphasize the importance of considering the type of muscle when studying the action of myotoxic agents.
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EDL Mouse Soleus Ultrastructural changes Veratrine cevadine tetrodotoxin animal cell animal tissue cell organelle cell swelling cell ultrastructure controlled study correlation analysis extensor digitorum longus muscle fragmentation reaction injection micromorphology mitochondrion mouse muscle cell muscle contraction myofilament nonhuman premedication reaction analysis sarcolemma sarcomere sarcoplasmic reticulum sodium transport soleus muscle animal Bagg albino mouse chemically induced disorder drug antagonism drug effect fast muscle fiber male muscle disease pathology skeletal muscle slow muscle fiber ultrastructure Animalia Animals Drug Antagonism Male Mice Mice, Inbred BALB C Mitochondria Muscle Fibers, Fast-Twitch Muscle Fibers, Slow-Twitch Muscle, Skeletal Muscular Diseases Sarcomeres Tetrodotoxin
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Inglês
Citação
Journal of Submicroscopic Cytology and Pathology, v. 34, n. 3, p. 305-313, 2002.
