Publicação: First molecular characterization of Cryptosporidium spp. infecting buffalo calves in Brazil
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With the aim of determining the occurrence of Cryptosporidium spp., 222 fecal samples were collected from Murrah buffalo calves aged up to 6 mo. Fecal DNA was genotyped with a nested polymerase chain reaction targeting the 18S rRNA gene and sequencing of the amplified fragment. Nested 18S PCR was positive for 48.2% of the samples. Sequence analysis showed that the most frequent species in these animals was Cryptosporidium ryanae, which was present in buffalo calves as young as 5 d. The zoonotic species Cryptosporidium parvum was detected in one animal. An uncommon Cryptosporidium 18S genotype was found in buffaloes.
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Cryptosporidiosis, Cryptosporidium ryanae, Genotype, Nested-pcr, Water buffaloes
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Inglês
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The Journal Of Eukaryotic Microbiology, v. 62, n. 5, p. 657-661, 2015.
