Gut Content and Laboratory Survival of the Termite Cornitermes cumulans (Isoptera: Termitidae: Syntermitinae) with Different Diets Including Nest Stored Food

Abstract

Cornitermes cumulans (Kollar, 1832) is a mound-building termite that stores food nodules in the nest, which seems to be pre-chewed and regurgitated by workers. Information on the food resources used by this species is still scarce, and this study aimed to understand the feeding habits of C. cumulans using a qualitative analysis of the worker gut contents, and to select the best food to keep termites under laboratorial conditions. Thus, four food resources and five artificial diets were tested to evaluate the laboratory survival of C. cumulans. Food nodules from nests were used as a positive control, and no food was available in cages for negative control. Ten replicates using 30 workers plus three soldiers were performed for each type of food and for each control. The experiments were daily examined for termite survival during a 40-day period. Data were analyzed using the log-rank (Mantel-cox) test (P < 0.05). The qualitative analysis of the gut contents showed different materials, such as plant parts, fungi, and minerals. Data analysis showed that all of the survival curves of termites fed with artificial diets and food items were significantly different from that obtained with termites fed on food nodules, which showed the highest survival, followed by the diets of α-cellulose, corn stalk, and sugarcane bagasse. Then, our findings showed that the most adequate artificial foods to maintain populations of C. cumulans in the laboratory were the diets of α-cellulose, corn stalk, and sugarcane bagasse.