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Publicação:
Use of mucus as a non-invasive sampling method for DNA barcoding of stingrays and skates (batoid elasmobranchs)

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dc.contributor.authorDomingues, Rodrigo R.
dc.contributor.authorGarrone-Neto, Domingos [UNESP]
dc.contributor.authorHilsdorf, Alexandre W. S.
dc.contributor.authorGadig, Otto B. F. [UNESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionNúcleo Integrado de Biotecnologia
dc.date.accessioned2019-10-06T16:21:22Z
dc.date.available2019-10-06T16:21:22Z
dc.date.issued2019-03-01
dc.description.abstractIn this study we tested the use of mucus from five species of Neotropical marine batoid elasmobranchs to extract genomic DNA for barcoding and phylogenetic analysis. The DNA from all individuals sampled was successfully amplified and sequenced for molecular barcode, allowing 99–100% accuracy to the species level. This method proved to provide reliable and good-quality DNA for barcoding and phylogenetic analysis of Neotropical elasmobranchs, through rapid handling and with low disturbance to animals.en
dc.description.affiliationDepartamento de Ciências do Mar UNIFESP – Universidade Federal de São Paulo Instituto do Mar
dc.description.affiliationUNESP – Universidade Estadual Paulista Campus Experimental de Registro
dc.description.affiliationUNESP – Universidade Estadual Paulista Campus do Litoral Paulista Instituto de Biociências Laboratório de Pesquisa de Elasmobrânquios
dc.description.affiliationUMC – Universidade de Mogi das Cruzes Núcleo Integrado de Biotecnologia
dc.description.affiliationUnespUNESP – Universidade Estadual Paulista Campus Experimental de Registro
dc.description.affiliationUnespUNESP – Universidade Estadual Paulista Campus do Litoral Paulista Instituto de Biociências Laboratório de Pesquisa de Elasmobrânquios
dc.format.extent512-516
dc.identifierhttp://dx.doi.org/10.1111/jfb.13919
dc.identifier.citationJournal of Fish Biology, v. 94, n. 3, p. 512-516, 2019.
dc.identifier.doi10.1111/jfb.13919
dc.identifier.issn1095-8649
dc.identifier.issn0022-1112
dc.identifier.scopus2-s2.0-85063145356
dc.identifier.urihttp://hdl.handle.net/11449/188856
dc.language.isoeng
dc.relation.ispartofJournal of Fish Biology
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectcytochrome c oxidase subunit 1
dc.subjectendangered elasmobranchs
dc.subjectmolecular markers
dc.subjectnon-lethal methods
dc.titleUse of mucus as a non-invasive sampling method for DNA barcoding of stingrays and skates (batoid elasmobranchs)en
dc.typeArtigo
dspace.entity.typePublication
unesp.author.orcid0000-0002-8979-645X[1]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, São Vicentept
unesp.departmentEngenharia Agronômica - FCAVRpt
unesp.departmentCiências Biológicas - IBCLPpt

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Coleções

Registro - FCAVR - Faculdade de Ciências Agrárias do Vale do Ribeira
São Vicente - IBCLP - Instituto de Biociências