Isolation and characterization of eight microsatellite loci in Leporinus macrocephalus (Characiformes : Anostomidae) and cross-species amplification
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Undergraduate course
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Blackwell Publishing
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Article
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Abstract
Leporinus macrocephalus is a species endemic to the Paraguay River basin and an important fishery resource, as well as a valuable species in aquaculture programs. A total of eight polymorphic microsatellite loci were isolated and characterized. A population survey was conducted involving 45 specimens whereby a large number of alleles (range 5-17 among loci), a highly observed (0.1667-0.6129) and an expected (0.6967-0.9448) heterozygosity was detected, indicating its usefulness in population genetics studies. Cross-species amplification was successful in eight Characiformes species.
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Keywords
aquaculture, cross-amplification, evolution, fish, microsatellite isolation, repetitive sequences, Leporinus macrocephalus, microsatellite
Language
English
Citation
Molecular Ecology Notes. Oxford: Blackwell Publishing, v. 7, n. 1, p. 32-34, 2007.
