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In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells

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dc.contributor.authorPereira-Junior, Oduvaldo Câmara Marques
dc.contributor.authorRahal, Sheila Canevese [UNESP]
dc.contributor.authorLima-Neto, João Ferreira
dc.contributor.authorLandim-Alvarenga, Fernanda da Cruz [UNESP]
dc.contributor.authorMonteiro, Frederico Ozanan Barros
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFederal Rural University of the Amazon Institute of Animal Health and Production
dc.date.accessioned2014-05-20T15:14:03Z
dc.date.available2014-05-20T15:14:03Z
dc.date.issued2013-05-01
dc.description.abstractPURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.en
dc.description.affiliationUNESP School of Veterinary Medicine and Animal Science Department of Veterinary Surgery and Anesthesiology
dc.description.affiliationUNESP School of Veterinary Medicine and Animal Department of Animal Reproduction and Veterinary Radiology
dc.description.affiliationFederal Rural University of the Amazon Institute of Animal Health and Production
dc.description.affiliationUnespUNESP School of Veterinary Medicine and Animal Science Department of Veterinary Surgery and Anesthesiology
dc.description.affiliationUnespUNESP School of Veterinary Medicine and Animal Department of Animal Reproduction and Veterinary Radiology
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent353-360
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502013000500006
dc.identifier.citationActa Cirúrgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 5, p. 353-360, 2013.
dc.identifier.doi10.1590/S0102-86502013000500006
dc.identifier.fileS0102-86502013000500006.pdf
dc.identifier.issn0102-8650
dc.identifier.lattes1497433265390194
dc.identifier.orcid0000-0002-9211-4093
dc.identifier.scieloS0102-86502013000500006
dc.identifier.urihttp://hdl.handle.net/11449/29025
dc.identifier.wosWOS:000319994200006
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirúrgica Brasileira
dc.relation.ispartofjcr0.933
dc.relation.ispartofsjr0,395
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectHydroxyapatitesen
dc.subjectBone Matrixen
dc.subjectCastor Oilen
dc.subjectPolyurethanesen
dc.subjectStem Cellsen
dc.titleIn vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cellsen
dc.typeArtigo
dspace.entity.typePublication
unesp.author.lattes1497433265390194
unesp.author.orcid0000-0002-1406-9979[5]
unesp.author.orcid0000-0002-9211-4093[2]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentCirurgia e Anestesiologia Veterinária - FMVZpt

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Botucatu - FMVZ - Faculdade de Medicina Veterinária e Zootecnia