Publicação:
Lignases Production and Partial Purification of Mnp from Brazilian Fungal Isolate in Submerged Fermentation

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dc.contributor.authorMarti, Ferhan
dc.contributor.authorLeao, Alcides Lopes [UNESP]
dc.contributor.authorMelo, Itamar S de
dc.contributor.authorYan, Norman D.
dc.contributor.authorSain, Mohini
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Toronto
dc.contributor.institutionEmbrapa Environment
dc.date.accessioned2016-04-01T18:45:13Z
dc.date.available2016-04-01T18:45:13Z
dc.date.issued2012
dc.description.abstractThe potential of ligninases as a green tool for effective valorization of lignin can be shown through enzymatic cocktails containing different lignin degrading enzymes. The present study deals with the screening of potential fungal strains useful for the liquefaction of bark containing lignin. Three different local isolates (Pleurotus ostreatus POS97/14, Pycnoporus sanguineus and the local isolated fungal strain) were selected out of ten different strains for ligninases production. Maximum production of enzymes was observed in the local isolated fungal strain after ten days in submerged fermentation. The isolated fungal strain produces ligninases mainly for manganese peroxidase (MnP). The enzyme oxidized a variety of the usual MnP substrates, including lignin related phenols. Furthermore, the partial purification for MnP was determined by FPLC and the molecular weight was evaluated by SDS-PAGE.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Departamento de Ciências Ambientais, Faculdade de Ciências Agronômicas de Botucatu, Botucatu, Rua Doutor José Barbosa de Barros, 1.780, Jardim Paraíso, CEP 18610307, SP, Brasil
dc.description.affiliationCenter for Biocomposites and Biomaterials Processing, Faculty of Forestry, University of Toronto, Toronto, ON, Canada
dc.description.affiliationDepartment of Microbiology, Embrapa Environment, Jaguariúna, SP, Brazil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Departamento de Ciências Ambientais, Faculdade de Ciências Agronômicas de Botucatu, Botucatu, Rua Doutor José Barbosa de Barros, 1.780, Jardim Paraíso, CEP 18610307, SP, Brasil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent1-7
dc.identifierhttp://dx.doi.org/10.4172/2167-7972.1000106
dc.identifier.citationFermentation Technology, v. 1, n. 5, p. 1-7, 2012.
dc.identifier.doi10.4172/2167-7972.1000106
dc.identifier.issn2167-7972
dc.identifier.urihttp://hdl.handle.net/11449/137329
dc.language.isoeng
dc.relation.ispartofFermentation Technology
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectLigninasesen
dc.subjectFungal strainen
dc.subjectPleurotus ostreatusen
dc.subjectP. sanguineusen
dc.subjectSubmerged fermentation, Fast protein liquid chromatographyen
dc.subjectManganese peroxidaseen
dc.subjectLignin peroxidaseen
dc.subjectLccen
dc.subjectTime course studiesen
dc.titleLignases Production and Partial Purification of Mnp from Brazilian Fungal Isolate in Submerged Fermentationen
dc.typeArtigo
dspace.entity.typePublication
unesp.author.orcid0000-0003-4381-8104[2]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Agronômicas, Botucatupt
unesp.departmentCiências Ambientaispt
unesp.departmentCiência Florestal - FCApt

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Botucatu - FCA - Faculdade de Ciências Agronômicas