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Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto

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dc.contributor.authorRusso-Carbolante, Elisa Maria de Sousa
dc.contributor.authorPenteado, Flora Cristina Lobo [UNESP]
dc.contributor.authorMedeiros, Luciene
dc.contributor.authorKashima, Simone
dc.contributor.authorTakayanagui, Oswaldo M.
dc.contributor.authorCovas, Dimas Tadeu
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:22:27Z
dc.date.available2014-05-27T11:22:27Z
dc.date.issued2007-05-01
dc.description.abstractHTLV-1 is the virus that causes T cell lymphoma/leukemia in adults and a neurological disorder known as HTLV-associated myelopathy or tropical spastic paraparesis. One of the transmission means is through contaminated blood and its byproducts. Because of the risk of HTLV-associated infections, screening for HTLV was introduced for Brazilian blood donors in 1993. Most of the diagnostic kits used in the national blood banks are bought from foreign companies. Brazil does not have the technology to produce this material and there is a need to produce diagnostic systems with national technology. In this study, we show the expression of gp21/HTLV-1 in Escherichia coli and its reactivity towards monoclonal antibodies and the antibodies of infected patients. Expressing these proteins is the first step towards obtaining diagnostic kits with Brazilian biotechnology.en
dc.description.affiliationCentro Regional de Hemoterapia Hospital Das Clínicas Universidade de São Paulo, Ribeirão Preto, SP
dc.description.affiliationDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas Júlio de Mesquita Filho Universidade Estadual Paulista, Araraquara, SP
dc.description.affiliationDepartamento de Neurologia Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, Ribeirão Preto, SP
dc.description.affiliationDepartamento de Clínica Médica Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, Ribeirão Preto, SP
dc.description.affiliationCentro Regional de Hemoterapia HCFMRP USP, R. Tenente Catão Roxo 2501, 14051-140 Ribeirão Preto, SP
dc.description.affiliationUnespDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas Júlio de Mesquita Filho Universidade Estadual Paulista, Araraquara, SP
dc.format.extent277-281
dc.identifierhttp://dx.doi.org/10.1590/S0037-86822007000300005
dc.identifier.citationRevista da Sociedade Brasileira de Medicina Tropical, v. 40, n. 3, p. 277-281, 2007.
dc.identifier.doi10.1590/S0037-86822007000300005
dc.identifier.file2-s2.0-34547161328.pdf
dc.identifier.issn0037-8682
dc.identifier.scieloS0037-86822007000300005
dc.identifier.scopus2-s2.0-34547161328
dc.identifier.urihttp://hdl.handle.net/11449/69639
dc.identifier.wosWOS:000248032900005
dc.language.isopor
dc.relation.ispartofRevista da Sociedade Brasileira de Medicina Tropical
dc.relation.ispartofjcr1.358
dc.relation.ispartofsjr0,658
dc.rights.accessRightsAcesso abertopt
dc.sourceScopus
dc.subjectgp21
dc.subjectHeterologous expression
dc.subjectHTLV-1
dc.subjectRecombinant proteins
dc.subjectmembrane protein
dc.subjectmonoclonal antibody
dc.subjectvirus antibody
dc.subjectvirus glycoprotein
dc.subjectantigen antibody reaction
dc.subjectbiotechnology
dc.subjectdiagnostic kit
dc.subjectEscherichia coli
dc.subjectHuman T cell leukemia virus 1
dc.subjectmolecular cloning
dc.subjectprokaryote
dc.subjectprotein expression
dc.subjectvirus diagnosis
dc.subjectAntibodies, Monoclonal
dc.subjectCloning, Molecular
dc.subjectenv Gene Products, Human Immunodeficiency Virus
dc.subjectGene Expression
dc.subjectGene Products, env
dc.subjectGenetic Vectors
dc.subjectHTLV-I Antibodies
dc.subjectHuman T-lymphotropic virus 1
dc.subjectHumans
dc.subjectImmunoblotting
dc.subjectPolymerase Chain Reaction
dc.subjectProkaryota
dc.titleClonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procariotopt
dc.title.alternativeCloning and expression of the transmembranic glycoprotein from human T cell lymphotropic virus in a prokaryotic systemen
dc.typeArtigopt
dcterms.licensehttp://www.scielo.br/revistas/rsbmt/paboutj.htm
dspace.entity.typePublication
relation.isDepartmentOfPublicationa83d26d6-5383-42e4-bb3c-2678a6ddc144
relation.isDepartmentOfPublication.latestForDiscoverya83d26d6-5383-42e4-bb3c-2678a6ddc144
relation.isOrgUnitOfPublication95697b0b-8977-4af6-88d5-c29c80b5ee92
relation.isOrgUnitOfPublication.latestForDiscovery95697b0b-8977-4af6-88d5-c29c80b5ee92
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentAnálises Clínicas - FCFpt

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