Publication:
Effects of ascorbic acid on in vitro culture of bovine preantral follicles

dc.contributor.authorAndrade, Evelyn R.
dc.contributor.authorVan Den Hurk, Robert
dc.contributor.authorLisboa, Lívia A.
dc.contributor.authorHertel, Mariana F.
dc.contributor.authorMelo-Sterza, Fabiana A.
dc.contributor.authorMoreno, Kleber
dc.contributor.authorBracarense, Ana Paula F.R.L.
dc.contributor.authorLandim-Alvarenga, Fernanda C. [UNESP]
dc.contributor.authorSeneda, Marcelo M.
dc.contributor.authorAlfieri, Amauri A.
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUtrecht University
dc.contributor.institutionUniversidade Estadual Do Mato Grosso Do sul
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:27:07Z
dc.date.available2014-05-27T11:27:07Z
dc.date.issued2012-11-01
dc.description.abstractThe objective of this study was to evaluate the effects of adding ascorbic acid to the media for in vitro culture of cattle ovarian fragments and to determine their effects on growth activation and viability of early-stage follicles. The ovarian cortex was divided into small fragments; one fragment was immediately fixed (control) and the other fragments were cultured in minimum essential medium (MEM) supplemented or not with various doses of ascorbic acid. Ovarian tissue was processed for histology, transmission electron microscopy (TEM) and immunohistochemical demonstration of proliferating cell nuclear antigen (PCNA). Compared with control fragments, the percentage of primordial follicles was reduced (p < 0.05) and the percentage of growing follicles had increased (p < 0.05) in cultured cortical fragments, independent of the tested medium or incubation time. Furthermore, compared with control tissue, culture of ovarian cortex for 8 days reduced the percentages of healthy, viable follicles (p < 0.05), but not when cultures were supplemented with 25, 50 or 100 μg/ml of ascorbic acid. Ultrastructural and immunohistochemical analysis of 8 day cultured ovarian cortical fragments, however, showed the integrity and viability of follicles only when fragments were cultured in presence of 50 μg/ml of ascorbic acid. In conclusion, this study demonstrated that addition of ascorbic acid to MEM at a concentration of 50 μg/ml not only stimulates the activation of 8 day in vitro cultured cattle primordial follicles and subsequent growth of activated follicles, but also safeguards the viability of these early-stage follicles. © 2012 Copyright Cambridge University Press.en
dc.description.affiliationUniversidade Estadual de Londrina, Londrina, PR
dc.description.affiliationFaculty of VeterinaryMedicine Utrecht University, Utrecht
dc.description.affiliationUniversidade Estadual Do Mato Grosso Do sul, Aquidauna, MS
dc.description.affiliationUniversidade Estadual de São Paulo, Botucatu, SP
dc.description.affiliationUnespUniversidade Estadual de São Paulo, Botucatu, SP
dc.format.extent379-388
dc.identifierhttp://dx.doi.org/10.1017/S0967199412000056
dc.identifier.citationZygote, v. 20, n. 4, p. 379-388, 2012.
dc.identifier.doi10.1017/S0967199412000056
dc.identifier.file2-s2.0-84867227314.pdf
dc.identifier.issn0967-1994
dc.identifier.issn1469-8730
dc.identifier.lattes8456490300814833
dc.identifier.orcid0000-0002-2420-2550
dc.identifier.scopus2-s2.0-84867227314
dc.identifier.urihttp://hdl.handle.net/11449/73703
dc.language.isoeng
dc.relation.ispartofZygote
dc.relation.ispartofjcr1.114
dc.relation.ispartofsjr0,387
dc.relation.ispartofsjr0,387
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAscorbic acid
dc.subjectCattle
dc.subjectIn vitro culture
dc.subjectPreantral follicles
dc.subjectascorbic acid
dc.subjectcycline
dc.subjectfollitropin
dc.subjectanimal
dc.subjectcattle
dc.subjectculture medium
dc.subjectdrug effect
dc.subjectfemale
dc.subjectmetabolism
dc.subjectovary follicle
dc.subjecttransmission electron microscopy
dc.subjectultrastructure
dc.subjectAnimals
dc.subjectAscorbic Acid
dc.subjectCulture Media
dc.subjectFemale
dc.subjectFollicle Stimulating Hormone
dc.subjectMicroscopy, Electron, Transmission
dc.subjectOvarian Follicle
dc.subjectProliferating Cell Nuclear Antigen
dc.subjectBos
dc.subjectBovinae
dc.titleEffects of ascorbic acid on in vitro culture of bovine preantral folliclesen
dc.typeArtigo
dcterms.licensehttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676
dspace.entity.typePublication
unesp.author.lattes8456490300814833[8]
unesp.author.orcid0000-0002-2420-2550[8]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentReprodução Animal e Radiologia Veterinária - FMVZpt

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