Screening of culture condition for xylanase production by filamentous fungi
dc.contributor.author | Garcia Simoes, Maria Lucia | |
dc.contributor.author | Tauk-Tornisielo, Samia Maria [UNESP] | |
dc.contributor.author | Tapia Tapia, Daniel Mario | |
dc.contributor.institution | Univ Estadual Sudoeste Bahia | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2013-09-30T18:48:35Z | |
dc.date.accessioned | 2014-05-20T13:57:45Z | |
dc.date.available | 2013-09-30T18:48:35Z | |
dc.date.available | 2014-05-20T13:57:45Z | |
dc.date.issued | 2009-11-16 | |
dc.description.abstract | The objective of this research was to investigate xylanase production by filamentous fungi (Trichoderma viride) to determine the best cultivation conditions in the process, aiming toward optimization of enzyme production. The best temperature, as well as the best carbon source, for biomass production was determined through an automated turbidimetric method (Bioscreen-C). The enzyme activity of this fungus was separately evaluated in two solid substrates (wheat and soybean bran) and in Vogel medium, pure and by adding other carbon sources. Temperature effects, cultivation time, and spore concentrations were also tested. The best temperature and carbon source for enzyme and biomass production was 25 C and sorbitol, respectively. Maximum xylanase activity was achieved when the fungus was cultivated in wheat bran along with sorbitol (1%, w/v), using a spore concentration of 2 x 10(6) spores. mL(-1), pH 5.0, for 144 h cultivation. The study demonstrated not only the importance of the nature of the substrate in obtaining a system resistant to catabolic repression, but also the importance of the culture conditions for biosynthesis of this enzyme. T. viride showed a high potential for xylanase production under the conditions presented in these assays. | en |
dc.description.affiliation | Univ Estadual Sudoeste Bahia, BR-45050020 Vitoria da Conquista, BA, Brazil | |
dc.description.affiliation | Univ Estadual Paulista, Ctr Estudos Ambientais, BR-13506900 Rio Claro, SP, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Ctr Estudos Ambientais, BR-13506900 Rio Claro, SP, Brazil | |
dc.description.sponsorship | Centro de Estudos Ambientais (CEA) at UNESP | |
dc.description.sponsorship | Postgraduate Course in Biological Sciences, Applied Microbiology, at the Rio Claro, SP, Campus of UNESP | |
dc.format.extent | 6317-6326 | |
dc.identifier | http://www.academicjournals.org/AJB/abstracts/abs2009/16Nov/Simoes%20et%20al.htm | |
dc.identifier.citation | African Journal of Biotechnology. Victoria Island: Academic Journals, v. 8, n. 22, p. 6317-6326, 2009. | |
dc.identifier.file | WOS000273718900049.pdf | |
dc.identifier.issn | 1684-5315 | |
dc.identifier.uri | http://hdl.handle.net/11449/20581 | |
dc.identifier.wos | WOS:000273718900049 | |
dc.language.iso | eng | |
dc.publisher | Academic Journals | |
dc.relation.ispartof | African Journal of Biotechnology | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.subject | Trichoderma viride | en |
dc.subject | xylanase activity | en |
dc.subject | enzyme optimization | en |
dc.title | Screening of culture condition for xylanase production by filamentous fungi | en |
dc.type | Artigo | |
dcterms.license | http://www.academicjournals.org/Creative%20Common%20Attribution%20License.htm | |
dcterms.rightsHolder | Academic Journals | |
dspace.entity.type | Publication |
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