Publicação: Coconut Water as an Extender Component for Cooled Equine Sperm
Carregando...
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Tipo
Artigo
Direito de acesso
Acesso aberto
Resumo
The aim of this study was to evaluate the effect of coconut water as a component of extender in different formulations for cooling equine sperm. One ejaculate of fourteen stallions was collected. Sperm was diluted to 50 x 10(6) sperm/mL using five different extenders: ACP-105: powdered coconut water extender (ACP-105, ACP Biotecnologia, Brazil); ACP-Milk: ACP-105 + 20 g/L of skimmed milk; ACP-EY 2.5%: ACP105 + 2.5% of egg yolk; ACP-EY 5%: ACP-105 + 5% of egg yolk; and BotuSemen (Botupharma, Botucatu, Brazil) and cooled in passive cooling device (BotuFlex, Botupharma, Botucatu, Brazil) at 5 and 15 degrees C for 24 hours. Sperm kinetics and plasma membrane integrity (PMI) were evaluated by computer-assisted sperm analysis and fluorescence staining, respectively, at TO (before cooling) and T24 (24 hours after cooling). Sperm kinetics did not differ at TO among groups (P > .05); however, at T24, these parameters were significantly lower in ACP-105 (5 degrees C, total motility [TM]: 9.2 +/- 3.6%; progressive motility [PM]: 2.7 +/- 1.6%; percentage of fast-moving spermatozoa [RAP]: 4.8 +/- 3.0%; 15 degrees C, TM: 10.6 +/- 3.0%; PM: 1.1 +/- 0.5%; RAP: 4.8 +/- 1.9%) and ACP-EY 5% (5 degrees C, TM: 28.0 +/- 6.3%; PM: 5.7 +/- 1.8%; RAP: 15.9 +/- 6.0%; 15 degrees C, TM: 30.0 +/- 6.0%; PM: 6.9 +/- 2.1%; RAP: 17.6 +/- 5.3%) compared with BotuSemen (5 degrees C, TM: 66.2 +/- 5.6%; PM: 21.1 +/- 2.8%; RAP: 53.9 +/- 6.1%; 15 degrees C, TM: 63.4 +/- 5.4%; PM: 17.2 +/- 2.8%; RAP: 51.4 +/- 6.3%) (P < .05). All groups exhibited similar PMI at tested moments and cooling temperatures (5 degrees C: 83%; 15 degrees C: 84%) (P> .05). Further studies are necessary to evaluate powdered coconut water in different compositions of sperm extender; however, coconut-based extender as used in this study was not an alternative to preserve sperm parameters of cooled equine sperm. (C) 2019 Elsevier Inc. All rights reserved.
Descrição
Palavras-chave
Semen, stallion, equine reproduction, fertility
Idioma
Inglês
Como citar
Journal Of Equine Veterinary Science. New York: Elsevier Science Inc, v. 78, p. 69-73, 2019.
