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Metachromasia for blood basophils identification in hybrid surubim catfish: Methodological contribution

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dc.contributor.authorDe Pádua, Santiago Benites [UNESP]
dc.contributor.authorIshikawa, Márcia Mayumi
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2022-04-29T07:13:57Z
dc.date.available2022-04-29T07:13:57Z
dc.date.issued2011-01-01
dc.description.abstractDifferent protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason.en
dc.description.affiliationCentro de Aquicultura da Unesp (CAUNESP), Rod. Paulo Donato Castellane, Jaboticabal, SP 14884-900
dc.description.affiliationEmbrapa Agropecuária Oeste, BR 163, Caixa Postal 661, Dourados, MS 79804-970
dc.description.affiliationUnespCentro de Aquicultura da Unesp (CAUNESP), Rod. Paulo Donato Castellane, Jaboticabal, SP 14884-900
dc.format.extent147-150
dc.identifier.citationRevista Brasileira de Medicina Veterinaria, v. 33, n. 3, p. 147-150, 2011.
dc.identifier.issn2527-2179
dc.identifier.issn0100-2430
dc.identifier.scopus2-s2.0-84894299439
dc.identifier.urihttp://hdl.handle.net/11449/227576
dc.language.isopor
dc.relation.ispartofRevista Brasileira de Medicina Veterinaria
dc.sourceScopus
dc.subjectBasophils marking
dc.subjectCytochemistry
dc.subjectFish hematology
dc.subjectToluidine blue
dc.titleMetachromasia for blood basophils identification in hybrid surubim catfish: Methodological contributionen
dc.titleMetacromasia para identificação de basófilos sanguíneos em surubim híbrido: Contribuição metodológicapt
dc.typeArtigo
dspace.entity.typePublication

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Jaboticabal - CAUNESP - Centro de Aquicultura da Unesp