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Genotypic Trypanosoma cruzi distribution and parasite load differ ecotypically and according to parasite genotypes in Triatoma brasiliensis from endemic and outbreak areas in Northeastern Brazil

dc.contributor.authorValença-Barbosa, Carolina
dc.contributor.authorFinamore-Araujo, Paula
dc.contributor.authorMoreira, Otacilio C.
dc.contributor.authorVergara-Meza, Jose Gabriel
dc.contributor.authorAlvarez, Marcus Vinicius Niz [UNESP]
dc.contributor.authorNascimento, Juliana R.
dc.contributor.authorBorges-Veloso, André
dc.contributor.authorViana, Maria Carolina
dc.contributor.authorLilioso, Maurício
dc.contributor.authorMiguel, Danilo Ciccone
dc.contributor.authorGadelha, Fernanda Ramos
dc.contributor.authorTeixeira, Marta Maria Geraldes
dc.contributor.authorAlmeida, Carlos Eduardo
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionFundação Oswaldo Cruz – Fiocruz
dc.contributor.institutionInstituto Oswaldo Cruz/Fiocruz
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal Fluminense (UFF)
dc.contributor.institutionUniversidade Federal da Bahia (UFBA)
dc.date.accessioned2022-04-28T19:42:07Z
dc.date.available2022-04-28T19:42:07Z
dc.date.issued2021-10-01
dc.description.abstractThis study aimed to identify the Trypanosoma cruzi genotypes and their relationship with parasitic load in distinct geographic and ecotypic populations of Triatoma brasiliensis in two sites, including one where a Chagas disease (ChD) outbreak occurred in Rio Grande do Norte state, Brazil. Triatomine captures were performed in peridomestic and sylvatic ecotopes in two municipalities: Marcelino Vieira – affected by the outbreak; and Currais Novos – where high pressure of peridomestic triatomine infestation after insecticide spraying have been reported. The kDNA-PCR was used to select 124 T. cruzi positive triatomine samples, of which 117 were successfully genotyped by fluorescent fragment length barcoding (FFLB). Moreover, the T. cruzi load quantification was performed using a multiplex TaqMan qPCR. Our findings showed a clear ecotypic segregation between TcI and TcII harboured by T. brasiliensis (p<0.001). Although no genotypes were ecotypically exclusive, TcI was predominant in peridomestic ecotopes (86%). In general, T. brasiliensis from Rio Grande do Norte had a higher T. cruzi load varying from 3.94 to 7.66 x 106 T. cruzi per insect. Additionally, TcII (median value=299,504 T. cruzi/intestine unit equivalents) had more than twice (p=0.1) the parasite load of TcI (median value=149,077 T. cruzi/intestine unit equivalents), which can be attributed to a more ancient co-evolution with T. brasiliensis. The higher prevalence of TcII in the sylvatic T. brasiliensis (70%) could be associated with a more diversified source of bloodmeals for wild insect populations. Either TcI or TcII may have been responsible for the ChD outbreak that occurred in the city of Marcelino Vieira. On the other hand, a smaller portion of T. brasiliensis was infected by TcIII (3%) in the peridomicile, in addition to T. rangeli genotype A (1%), often found in mixed infections. Our results highlight the need of understanding the patterns of T. cruzi genotype´s development and circulation in insect vectors and reservoirs as a mode of tracking situations of epidemiologic importance, as the ChD outbreak recently recorded for Northeastern Brazil.en
dc.description.affiliationInstituto de Biologia Universidade de Campinas – UNICAMP
dc.description.affiliationGrupo Triatomíneos Instituto René Rachou Fundação Oswaldo Cruz – Fiocruz
dc.description.affiliationLaboratório de Biologia Molecular e Doenças Endêmicas Instituto Oswaldo Cruz/Fiocruz
dc.description.affiliationInstituto de Ciências Biomédicas Universidade de São Paulo-USP
dc.description.affiliationInstituto de Biotecnologia de Botucatu Universidade Estadual Paulista
dc.description.affiliationInstituto de Química Universidade Federal Fluminense
dc.description.affiliationInstituto de Biologia Universidade Federal da Bahia
dc.description.affiliationUnespInstituto de Biotecnologia de Botucatu Universidade Estadual Paulista
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipIdCAPES: 165756/2018-7
dc.description.sponsorshipIdFAPESP: 2016/08176-9
dc.description.sponsorshipIdFAPESP: 2017-19420-0
dc.description.sponsorshipIdCAPES: 306357/2019-4
dc.description.sponsorshipIdCAPES: 308411/2017-0
dc.description.sponsorshipIdFAPERJ: JCNE- E-26/203.031/2018
dc.identifierhttp://dx.doi.org/10.1016/j.actatropica.2021.106054
dc.identifier.citationActa Tropica, v. 222.
dc.identifier.doi10.1016/j.actatropica.2021.106054
dc.identifier.issn1873-6254
dc.identifier.issn0001-706X
dc.identifier.scopus2-s2.0-85111241028
dc.identifier.urihttp://hdl.handle.net/11449/222052
dc.language.isoeng
dc.relation.ispartofActa Tropica
dc.sourceScopus
dc.subjectEpidemiology
dc.subjectGenotyping Trypanosoma cruzi
dc.subjectLineages, oral outbreaks
dc.subjectTriatomines
dc.titleGenotypic Trypanosoma cruzi distribution and parasite load differ ecotypically and according to parasite genotypes in Triatoma brasiliensis from endemic and outbreak areas in Northeastern Brazilen
dc.typeArtigopt
dspace.entity.typePublication
unesp.author.orcid0000-0001-9579-8710 0000-0001-9579-8710[1]
unesp.author.orcid0000-0003-1959-0373[4]
unesp.author.orcid0000-0001-7104-3954[5]
unesp.author.orcid0000-0003-2325-2684[6]
unesp.author.orcid0000-0003-4702-829X[9]
unesp.author.orcid0000-0003-0522-6562[10]
unesp.author.orcid0000-0002-1075-8830[11]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biotecnologia, Botucatupt

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